酿酒酵母中涉及CYC7-H3突变的基因转换事件的频率和方向性
Frequency and directionality of gene conversion events involving the CYC7-H3 mutation in Saccharomyces cerevisiae.
作者信息
Pukkila P J, Stephens M D, Binninger D M, Errede B
出版信息
Genetics. 1986 Oct;114(2):347-61. doi: 10.1093/genetics/114.2.347.
Abstract
The CYC7-H3 mutation is a 5-kb deletion that causes overproduction of iso-2 cytochrome c. Unlike most mutations in yeast, the CYC7-H3 mutation is preferentially lost when it is involved in a gene conversion event. We have shown that cloned copies of CYC7-H3 DNA that are inserted into the yeast genome are associated with a high frequency of recombination and aberrant segregation events. Since parity in conversion frequency was observed when the extensive insertion/deletion heterozygosity at this locus was eliminated, we conclude that the CYC7-H3 sequences are inherently capable of acting as donors or recipients in gene conversion events, although they are unlikely to act as donors when they are located opposite a large heterology. DNA sequence comparisons revealed similarities between the CYC7-H3 junction region and the 2-micron circle DNA region that is involved in site-specific recombination.
摘要
CYC7-H3突变是一个5千碱基的缺失，它会导致异-2细胞色素c过量产生。与酵母中的大多数突变不同，CYC7-H3突变在参与基因转换事件时优先丢失。我们已经表明，插入酵母基因组的CYC7-H3 DNA克隆拷贝与高频重组和异常分离事件相关。由于当该位点广泛的插入/缺失杂合性被消除时观察到转换频率的均等性，我们得出结论，CYC7-H3序列在基因转换事件中本质上能够作为供体或受体，尽管当它们位于与大的异源序列相对时不太可能作为供体。DNA序列比较揭示了CYC7-H3连接区域与参与位点特异性重组的2微米环状DNA区域之间的相似性。
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