Toki T, Oikawa N, Tase T, Satoh S, Wada Y, Yajima A
Gynecol Obstet Invest. 1986;22(2):97-101. doi: 10.1159/000298898.
Biopsies from 30 cases of vulvar condyloma, 460 cases of cervical dysplasia, 30 cases of carcinoma in situ and 75 cases of invasive carcinoma of the cervix were screened for the presence of human papillomavirus (HPV) antigen by means of the peroxidase-antiperoxidase method. Positive reaction for HPV was detected in 14 cases of condyloma and 80 cases of dysplasia as a brown intranuclear precipitate in the superficial layer of the epithelium. None of the cases of carcinoma in situ and invasive cancer were positive for HPV. The mean age of the women with HPV-positive dysplasia was significantly lower than that of the women with HPV-negative dysplasia. Condylomatous dysplasia showed a significantly higher positive rate than did noncondylomatous dysplasia. Cases of condylomatous dysplasia with severe stromal inflammation were negative for HPV more frequently than those with mild stromal inflammation.
采用过氧化物酶-抗过氧化物酶法，对30例外阴尖锐湿疣、460例宫颈发育异常、30例原位癌和75例宫颈浸润癌的活检标本进行人乳头瘤病毒（HPV）抗原检测。在14例尖锐湿疣和80例发育异常病例中检测到HPV阳性反应，表现为上皮表层细胞核内棕色沉淀。原位癌和浸润癌病例均未检测到HPV阳性。HPV阳性发育异常女性的平均年龄显著低于HPV阴性发育异常女性。湿疣样发育异常的阳性率显著高于非湿疣样发育异常。伴有严重间质炎症的湿疣样发育异常病例HPV阴性的频率高于伴有轻度间质炎症的病例。
