Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
Nano-Cosmeceuticals Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
J Photochem Photobiol B. 2018 Nov;188:50-59. doi: 10.1016/j.jphotobiol.2018.08.022. Epub 2018 Sep 1.
Bombyx mori silk extracts, derived from the cocoon degumming process of draw and dye silk in the textile industry, are mainly composed of sericin protein. To add value to the Thai silk extracts, and hence the silk industry, a simple enrichment process was recently developed and the enriched silk extracts were then applied in nano-cosmeceutical products and nano-delivery systems. In this study, the protective effect of Thai silk extracts from three different strains of Bombyx mori on the drug-induced phototoxicity was evaluated in vitro using chlorpromazine (CPZ), a commonly used antipsychotic drug, as a representative phototoxic drug. The human epidermal A431 cell line and reconstructed human epidermis (RhE) model were used as the in vitro skin model. The silk extracts significantly improved the viability of A431 cells after CPZ exposure and ultraviolet A (UVA) irradiation, as shown by the significantly increased CPZ and UVA IC values and the decreased proportion of apoptotic cells. The protective effect of these silk extracts against the CPZ-induced UVA-phototoxicity in A431 cells was associated with the attenuation of intracellular oxidative stress via an increased intracellular glutathione level. Likewise, the silk extracts exhibited a protective effect on the CPZ-induced UVA-phototoxicity in the RhE model, in terms of an improved tissue viability and attenuation of the released inflammatory cytokine, interleukin-1α. These findings support the potential usefulness of silk extracts in novel applications, especially in the protection of drug-induced phototoxicity.
家蚕丝素蛋白提取物来源于纺织工业中丝的拉伸和染色脱胶过程，主要由丝胶蛋白组成。为了提高泰国丝素蛋白提取物的附加值，从而提升丝绸产业的价值，最近开发了一种简单的富集工艺，然后将富集的丝素蛋白提取物应用于纳米化妆品和纳米给药系统。在这项研究中，使用氯丙嗪（CPZ）作为代表性光毒性药物，评估了来自三种不同家蚕品系的泰国丝素蛋白提取物对药物诱导的光毒性的体外保护作用。使用人表皮 A431 细胞系和重建的人体表皮（RhE）模型作为体外皮肤模型。丝素蛋白提取物显著提高了 CPZ 暴露和紫外线 A（UVA）照射后 A431 细胞的活力，这表现为 CPZ 和 UVA 的 IC 值显著增加，凋亡细胞的比例降低。这些丝素蛋白提取物对 A431 细胞中 CPZ 诱导的 UVA 光毒性的保护作用与通过增加细胞内谷胱甘肽水平来减轻细胞内氧化应激有关。同样，丝素蛋白提取物对 RhE 模型中 CPZ 诱导的 UVA 光毒性也表现出保护作用，表现为组织活力提高和释放的炎症细胞因子白细胞介素-1α 减少。这些发现支持丝素蛋白提取物在新应用中的潜在用途，特别是在保护药物诱导的光毒性方面。
