Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14049-900, Brazil.
Molecules. 2018 Sep 13;23(9):2339. doi: 10.3390/molecules23092339.
ArtinM, a d-mannose-binding lectin from , activates antigen-presenting cells by recognizing Toll-like receptor (TLR)2 and cluster of differentiation (CD)14 -glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4⁺ T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells.
ArtinM 是一种来自 的 D-甘露糖结合凝集素，通过识别 Toll 样受体 (TLR)2 和分化群 (CD)14-聚糖激活抗原呈递细胞，诱导细胞因子产生，并促进 1 型 T 辅助 (Th1) 免疫，这一过程在对抗真菌感染中起着辅助作用。我们最近证明 ArtinM 通过与 CD3 的直接相互作用刺激 CD4+T 细胞产生白细胞介素 (IL)-17。在这里，我们进一步研究了 ArtinM 对 B 细胞活化产生 IL-17 的影响。我们表明 ArtinM 激活了小鼠 B 细胞，增加了 IL-17 和 IL-12p40 的产生。ArtinM 的直接作用足以诱导 B 细胞产生 IL-17，并且我们没有发现在 ArtinM 存在下，来自 TLR2 或 CD14 敲除 (KO) 小鼠的 B 细胞与野生型 (WT) 小鼠的 B 细胞之间的 IL-17 水平存在差异。因此，ArtinM 通过碳水化合物识别对脾 B 细胞的影响可能有助于 Th17 免疫；然而，所涉及的机制与 ArtinM 与 TLR2 和 CD14 的相互作用无关。目前的工作代表了在理解凝集素在 B 细胞中诱导 IL-17 方面的开创性努力。
