阿廷 M：生物活性的纯化和评估。

ArtinM: Purification and Evaluation of Biological Activities.

机构信息

Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto School of Medicine University of São Paulo (FMRP/USP), Ribeirão Preto, SP, Brazil.

出版信息

Methods Mol Biol. 2020;2132:349-358. doi: 10.1007/978-1-0716-0430-4_34.

DOI:10.1007/978-1-0716-0430-4_34

PMID:32306342

Abstract

The immunomodulatory activity of plant lectins has been evaluated because of their high selectivity for glycans linked to receptors on innate and adaptative immune cells. ArtinM is a mannosyl-binding lectin, obtained from the seeds of Artocarpus heterophyllus, that induces the differentiation of CD4 T cells and macrophages by interacting with CD3 and TLR2/CD14, respectively. This ArtinM property ultimately favors the combat of intracellular pathogens, opening new perspectives on the lectins application as immunomodulatory agents. The current section describes protocols for purification and evaluation of ArtinM biological activity. The purification is based on the ArtinM-D-mannose affinity. The effect of inducing IL-12 production by murine macrophages cell line is adopted to evaluate the ArtinM biological activity.

摘要

由于植物凝集素对与先天和适应性免疫细胞上的受体相连的聚糖具有高度选择性，因此评估了其免疫调节活性。ArtinM 是一种甘露糖结合凝集素，从 Artocarpus heterophyllus 的种子中获得，通过分别与 CD3 和 TLR2/CD14 相互作用，诱导 CD4 T 细胞和巨噬细胞的分化。ArtinM 的这种特性最终有利于对抗细胞内病原体，为凝集素作为免疫调节剂的应用开辟了新的前景。本节描述了 ArtinM 生物活性的纯化和评估方案。该纯化基于 ArtinM-D-甘露糖的亲和力。采用诱导小鼠巨噬细胞系产生 IL-12 的方法来评估 ArtinM 的生物活性。