Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran.
Caspian Faculty of Engineering, College of Engineering, University of Tehran, P. O. Box: 119-43841, Rezvanshahr, Iran.
Int J Biol Macromol. 2019 Feb 1;122:1008-1016. doi: 10.1016/j.ijbiomac.2018.09.046. Epub 2018 Sep 11.
Poly (l-lactide-co-D, l-lactide) (PLDLLA) is a biodegradable polymer predominantly used in biomedical applications. Despite unprecedented characteristics of PLDLLA, its wettability, mechanical properties, degradation, and cell attachment are main issues to improve. In this work, different blend films based on PLDLLA/poly (acrylic acid) (PAAc) are prepared to evaluate their miscibility, hydrophilicity, hydrolytic degradation and mechanical properties. For this purpose, a series of experiments such as DSC alongside SEM, water contact angle (WCA)/water up-take, weight measurements in phosphate buffer saline (PBS) and NaOH as well as tensile test are carried out. The DSC and SEM results show a miscibility for the blends, and hence by increasing PAAc, the WCA values and degradation rates are decreased and increased, respectively. Moreover, the degradation mechanisms of the blend samples follow surface/bulk erosion and bulk process in the alkaline and PBS environments, respectively. Subsequently, PLDLLA and its blends are electrospun to prepare nanofibrous samples, thereby assessing their cytotoxicity and cell viability by the use of thiazolyl blue assay and acridine orange/ethidium bromide staining, respectively. The in vitro SNL 76/7 fibroblast cells cultivation onto the surface of the blend with 10% wt. of PAAc revealed that this sample is a promising candidate for tissue engineering applications.
聚（L-丙交酯-co-D，L-丙交酯）（PLDLLA）是一种可生物降解的聚合物，主要用于生物医学应用。尽管 PLDLLA 具有前所未有的特性，但它的润湿性、机械性能、降解和细胞附着仍然是需要改进的主要问题。在这项工作中，制备了不同的基于 PLDLLA/聚（丙烯酸）（PAAc）的共混膜，以评估它们的混溶性、亲水性、水解降解和机械性能。为此，进行了一系列实验，如 DSC 结合 SEM、水接触角（WCA）/吸水率、磷酸盐缓冲盐水（PBS）和 NaOH 中的重量测量以及拉伸试验。DSC 和 SEM 结果表明共混物具有混溶性，因此随着 PAAc 的增加，WCA 值和降解速率分别降低和增加。此外，共混样品的降解机制分别遵循碱性和 PBS 环境中的表面/体相侵蚀和体相过程。随后，通过静电纺丝制备了 PLDLLA 及其共混物的纳米纤维样品，通过噻唑蓝法和吖啶橙/溴化乙锭染色分别评估了它们的细胞毒性和细胞活力。体外 SNL 76/7 成纤维细胞在含有 10wt.%PAAc 的共混物表面的培养表明，该样品是组织工程应用的有前途的候选物。
