Lee F K, Pereira L, Griffin C, Reid E, Nahmias A
J Virol Methods. 1986 Sep;14(2):111-8. doi: 10.1016/0166-0934(86)90041-8.
A novel herpes simplex virus type 1 (HSV-1)-specific glycoprotein reactive with monoclonal antibody H1379 was purified by affinity chromatography. This glycoprotein, provisionally designated as gG-1, forms two sets of bands with molecular weights of 40-44,000 and 60-88,000. When used in an immunodot enzymatic assay, gG-1 reacted strongly with rabbit antisera to HSV-1, but not with sera hyperimmune to HSV-2. Specificity of the assay was further established by the lack of reactivity of convalescent sera collected from 20 patients with primary genital HSV-2 infections, and from 100 sero-negative individuals. In contrast, antibodies to gG-1 were detected in 9 of 10 patients with primary HSV-1 infection, and in 63/67 patients with culture-positive, recurrent oral or genital HSV-1 infection. Reproducibility of the gG-1 immunodot assay for HSV-1 antibody detection was 96%. Serological assay with purified gG-1, done in parallel with the assay using purified gG-2 described in an earlier report, provides simple and reliable methods to detect type-specific HSV-1 and HSV-2 antibodies for seroepidemiological studies.
通过亲和层析法纯化了一种与单克隆抗体H1379反应的新型单纯疱疹病毒1型（HSV-1）特异性糖蛋白。这种糖蛋白暂定为gG-1，形成两组分子量分别为40 - 44,000和60 - 88,000的条带。当用于免疫斑点酶测定时，gG-1与兔抗HSV-1血清强烈反应，但不与HSV-2超免疫血清反应。从20例原发性生殖器HSV-2感染患者和100例血清阴性个体收集的恢复期血清无反应性，进一步证实了该测定的特异性。相比之下，10例原发性HSV-1感染患者中有9例检测到gG-1抗体，67例培养阳性的复发性口腔或生殖器HSV-1感染患者中有63例检测到gG-1抗体。gG-1免疫斑点法检测HSV-1抗体的重现性为96%。用纯化的gG-1进行血清学检测，与早期报告中使用纯化的gG-2进行的检测平行进行，为血清流行病学研究提供了检测型特异性HSV-1和HSV-2抗体的简单可靠方法。
