通过酶免疫测定法检测巨细胞病毒抗体以及缺乏菌株异质性产生影响的证据。
Detection of cytomegalovirus antibody by enzyme immunoassay and lack of evidence for an effect resulting from strain heterogeneity.
作者信息
Adler S P, McVoy M
出版信息
J Clin Microbiol. 1986 Nov;24(5):870-2. doi: 10.1128/jcm.24.5.870-872.1986.
Abstract
A total of 259 serum samples from random blood donors were assayed by an enzyme immunoassay for antibody to cytomegalovirus (CMV) with antigens prepared from eight genetically unrelated clinical isolates of CMV and the laboratory-adapted strains AD169 and Towne. A total of 144 serum samples were reactive against all 10 strains, and 115 serum samples lacked immunoglobulin against all 10 strains. The results support the continued use of the AD169 strain of human CMV as the reference antigen for CMV serologic testing.
摘要
采用酶免疫分析法，使用从8株基因不相关的巨细胞病毒（CMV）临床分离株以及实验室适应株AD169和Towne制备的抗原，对259份随机献血者的血清样本进行了CMV抗体检测。共有144份血清样本对所有10株病毒呈反应性，115份血清样本对所有10株病毒均缺乏免疫球蛋白。结果支持继续使用人CMV的AD169株作为CMV血清学检测的参考抗原。
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