The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian Province, China.
College of Chemical Engineering and Materials Science, Quanzhou Normal University, Fujian Province, China.
Mikrochim Acta. 2018 Sep 14;185(10):458. doi: 10.1007/s00604-018-2989-x.
A composite consisting of graphene oxide and gold nanorods (GO-GNRs) was designed for the trace determination of hepatitis B surface antigen (HBsAg) using surface enhanced Raman spectroscopy (SERS). GO contains numerous carboxy and hydroxy groups on its surface and therefore can serve as the substrate for decoration with GNRs and for immobilizing antibody against HBsAg. The GNRs (carrying the SERS probe 2-mercaptopyridine) exhibit high SERS activity, and this improves the sensitivity of the biosensor. The antibody on the GO-GNRs binds HBsAg with high specificity, and it results in excellent selectivity. The SERS signal (measured at 1002 cm) increases in the 1-1000 pg·mL HBsAg concentrations range, and the limit of detection is 0.05 pg·mL (at an S/N ratio of 3). The immunoassay achieves the sensitive and selective determination of HBsAg in serum and expands the potential application of GO-GNR based SERS tag in clinical research. Graphical abstract A novel graphene oxide-gold nanorod (GO-GNRs) based surface-enhanced Raman scattering (SERS) tag for immunoassay was designed. It allows for sensitive and selective determination of HBsAg in serum. The method is expected to expand the potential application in the environment, in medicine and in food analysis.
一种由氧化石墨烯和金纳米棒（GO-GNRs）组成的复合材料被设计用于使用表面增强拉曼光谱（SERS）痕量测定乙型肝炎表面抗原（HBsAg）。GO 表面含有许多羧基和羟基，因此可以作为 GNRs 修饰和固定针对 HBsAg 的抗体的基底。金纳米棒（携带 SERS 探针 2-巯基吡啶）具有高 SERS 活性，这提高了生物传感器的灵敏度。GO-GNRs 上的抗体与 HBsAg 具有高特异性结合，从而具有出色的选择性。在 1-1000 pg·mL HBsAg 浓度范围内，SERS 信号（在 1002 cm 处测量）增加，检测限为 0.05 pg·mL（信噪比为 3）。免疫测定法实现了血清中 HBsAg 的灵敏和选择性测定，并扩展了基于 GO-GNR 的 SERS 标签在临床研究中的潜在应用。
