利用单倍体胚胎干细胞筛选参与X染色体失活的因子。

Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs.

作者信息

Monfort Asun, Di Minin Giulio, Wutz Anton

机构信息

Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, ETH Hönggerberg, Zurich, Switzerland.

出版信息

Methods Mol Biol. 2018;1861:1-18. doi: 10.1007/978-1-4939-8766-5_1.

DOI:10.1007/978-1-4939-8766-5_1

PMID:30218355

Abstract

Forward genetics can provide insight into molecular pathways as has been demonstrated by advances in cell biology from comprehensive genetic studies in simple organisms. Recently, techniques have become available that promise a similar potential for understanding developmental pathways in mammals. Here we describe a genetic forward screening approach for identifying factors involved in X chromosome inactivation that is based on haploid mouse embryonic stem cells. Using a genetically encoded selection system screening of large mutant pools can identify candidate silencing factors with high confidence.

摘要

正向遗传学能够深入了解分子途径，正如简单生物体中全面的遗传研究在细胞生物学领域所取得的进展所证明的那样。最近，出现了一些技术，有望在理解哺乳动物发育途径方面具有类似的潜力。在这里，我们描述了一种基于单倍体小鼠胚胎干细胞的用于鉴定参与X染色体失活的因子的遗传正向筛选方法。使用遗传编码的选择系统，对大型突变库进行筛选能够高度可靠地鉴定出候选沉默因子。