Hamblin C, Barnett I T, Crowther J R
J Immunol Methods. 1986 Oct 23;93(1):123-9. doi: 10.1016/0022-1759(86)90442-4.
The liquid-phase blocking sandwich ELISA has been evaluated for the serological study of antibodies against foot-and-mouth disease virus (FMDV). The titres recorded for sera from a population of more than 300 British uninfected, unvaccinated cattle which were examined against each of the seven immunologically distinct FMDV types were less than 1 in 40. A positive correlation between ELISA and VN titres was recorded for sera either vaccinated or involved in outbreaks of FMDV. The overall regression between the ELISA/VN data showed that 1 in 16 by VN was equivalent to 1 in 40 by ELISA. Thus ELISA is sensitive, specific and reproducible and results may be directly correlated to those recorded by VN. Serum titres may be interpreted as positive or negative and the number of sera which require retesting would be considerably less than by VN. It is suggested that this ELISA may be used as an alternative to the existing VN test for the quantification of antibodies against FMD virus.
已对液相阻断夹心酶联免疫吸附测定法（ELISA）进行评估，用于口蹄疫病毒（FMDV）抗体的血清学研究。对300多头未感染、未接种疫苗的英国牛群的血清进行检测，针对七种免疫特性不同的口蹄疫病毒类型中的每一种，记录的效价均低于40分之一。对于接种过疫苗或感染过口蹄疫病毒的血清，酶联免疫吸附测定法（ELISA）和病毒中和试验（VN）效价之间存在正相关。酶联免疫吸附测定法（ELISA）/病毒中和试验（VN）数据的总体回归表明，病毒中和试验（VN）的16分之一相当于酶联免疫吸附测定法（ELISA）的40分之一。因此，酶联免疫吸附测定法（ELISA）灵敏、特异且可重复，其结果可直接与病毒中和试验（VN）记录的结果相关联。血清效价可解释为阳性或阴性，需要重新检测的血清数量将大大少于病毒中和试验（VN）。建议这种酶联免疫吸附测定法（ELISA）可作为现有病毒中和试验（VN）的替代方法，用于定量口蹄疫病毒抗体。
