利用恒化器培养对诺尔斯链霉菌分化突变体进行定向筛选。
Directed selection of differentiation mutants of Streptomyces noursei using chemostat cultivation.
作者信息
Noack D
出版信息
J Basic Microbiol. 1986;26(4):231-9. doi: 10.1002/jobm.3620260412.
A nourseothricin-producing Streptomyces noursei strain was continuously cultivated in a chemostat equipped with a stirrer for mechanical fractionation of the mycelium. Different cultivation conditions allowed the selection of six types of differentiation mutants after the culture had reached a population genetically stationary state. The mutants showed an altered control pattern of sporulation as well as altered antibiotic biosynthesis and antibiotic resistance. In addition, the stability of the recombinant plasmid pIJ385 in several differentiation type mutants as host strains was tested. The results suggest that there exists a strong correlation between the cultivation conditions employed and the type of differentiation mutants selected.
一株产诺尔斯菌素的诺尔斯链霉菌菌株在配备搅拌器的恒化器中连续培养，用于菌丝体的机械分离。在培养物达到遗传稳定状态的群体后，不同的培养条件允许选择六种类型的分化突变体。这些突变体显示出孢子形成的控制模式改变，以及抗生素生物合成和抗生素抗性的改变。此外，还测试了重组质粒pIJ385在几种分化型突变体作为宿主菌株中的稳定性。结果表明，所采用的培养条件与所选分化突变体的类型之间存在很强的相关性。
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