Hänel F, Gräfe U, Friedrich W, Bormann E J
Z Allg Mikrobiol. 1984;24(4):239-45. doi: 10.1002/jobm.3630240407.
Mycelial levels of ATP and glucose-6-phosphate were investigated in mutants of streptothricin-producing S. noursei JA 3880b differing from the wild-type strain in antibiotic formation, in the control by inorganic phosphate of the secondary metabolism, and in the resistance to growth inhibition by toxic arsenate ions. As compared with the ancestral strain, mutants exhibited a lower content of ATP in the mycelium while addition of 0.1 M arsenate to growing cultures provoked only moderate changes in the level of this high-energy metabolite. The results suggest that there exists a correlation between growth resistance to arsenate and insensitivity to phosphate inhibition of the secondary metabolism, on the one hand, and the capacity to produce streptothricin-type antibiotics, on the other.
研究了产链丝菌素的诺尔斯链霉菌JA 3880b突变体菌丝体中ATP和6-磷酸葡萄糖的水平。这些突变体在抗生素形成、次级代谢的无机磷酸盐调控以及对有毒砷酸盐离子生长抑制的抗性方面与野生型菌株不同。与原始菌株相比，突变体菌丝体中ATP含量较低，而向生长培养物中添加0.1 M砷酸盐仅引起这种高能代谢物水平的适度变化。结果表明，一方面，对砷酸盐的生长抗性和对次级代谢磷酸盐抑制的不敏感性之间存在相关性，另一方面，与产生链丝菌素型抗生素的能力之间也存在相关性。
