Doddanna Sunitha Jagalur, Patel Shilpa, Sundarrao Madhusudan Astekar, Veerabhadrappa Ravindra Setru
Department of Oral Pathology and Microbiology, Teerthanker Mahaveer Institute of Dental Sciences and Research Centre, Teerthanker Mahaveer University, Bagarpur, Delhi Road, Moradabad, Uttar Pradesh, India.
Indian J Dent Res. 2013 Jul-Aug;24(4):401-5. doi: 10.4103/0970-9290.118358.
Plants as sources of medicinal compounds have continued to play a predominant role in the maintenance of human health since ancient times. Even though several effective antifungal agents are available for oral candida infections, the failure is not uncommon because isolates of Candida albicans may exhibits resistance to the drug during therapy. The present study was conducted to evaluate the antimicrobial effects of few plant extracts on Candida albicans. An additional objective was to identify an alternative, inexpensive, simple, and effective method of preventing and controlling Candida albicans.
Fine texture powder or paste form of leaves was soaked in sterile distilled water and 100% ethyl alcohol, which were kept in refrigerator at 4°C for 24 h. Then filtrates were prepared and kept in a hot air oven to get a black shining crystal powder/paste form. Stock solutions of plant extracts were inoculated on petri plates containing species of Candida albicans and incubated at 25 ± 2°C for 72 h.
Alcoholic curry leaves showed the maximum zone of inhibition on Candida albicans followed by aqueous tea leaves. The other plant extracts like alcoholic onion leaves, alcoholic tea leaves, alcoholic onion bulb, alcoholic aloe vera, and alcoholic mint leaves also inhibited the growth of Candida albicans but lesser extent.
The present study renders few medicinal plants as an alternative medicines to the field of dentistry which can be used adjunct to conventional therapy of oral candidasis.
自古以来，植物作为药用化合物的来源，在维护人类健康方面一直发挥着重要作用。尽管有几种有效的抗真菌药物可用于治疗口腔念珠菌感染，但治疗失败的情况并不罕见，因为白色念珠菌分离株在治疗过程中可能会对药物产生耐药性。本研究旨在评估几种植物提取物对白色念珠菌的抗菌作用。另一个目的是确定一种替代的、廉价、简单且有效的预防和控制白色念珠菌的方法。
将叶片的细粉或糊状物浸泡在无菌蒸馏水中和100%乙醇中，于4°C冰箱中保存24小时。然后制备滤液，并置于热风烘箱中，得到黑色闪亮的晶体粉末/糊状物。将植物提取物的储备液接种在含有白色念珠菌的培养皿上，于25±2°C孵育72小时。
酒精提取物的咖喱叶对白色念珠菌的抑菌圈最大，其次是水提取物的茶叶。其他植物提取物，如酒精提取物的洋葱叶、酒精提取物的茶叶、酒精提取物的洋葱鳞茎、酒精提取物的芦荟和酒精提取物的薄荷叶，也能抑制白色念珠菌的生长，但程度较小。
本研究表明，几种药用植物可作为牙科领域的替代药物，可用于辅助口腔念珠菌病的传统治疗。
