假型逆转录病毒载体揭示了大鼠细胞中对网状内皮组织增生症病毒复制的限制。
Pseudotyped retroviral vectors reveal restrictions to reticuloendotheliosis virus replication in rat cells.
作者信息
Embretson J E, Temin H M
出版信息
J Virol. 1986 Nov;60(2):662-8. doi: 10.1128/JVI.60.2.662-668.1986.
Abstract
Reticuloendotheliosis viruses (Rev) replicate in chicken and dog cells, but not in rat cells. Amphotropic murine leukemia viruses (Am-MLV) replicate in chicken, dog, and rat cells. Transcription from the Rev long terminal repeat, determined by the chloramphenicol acetyltransferase assay, was not significantly different from transcription from the MLV long terminal repeat in rat cells. To determine further the step(s) in the retroviral life cycle that is blocked for Rev replication in rat cells, we took advantage of the wide host range of Am-MLV (S. Rasheed, M. B. Gardner, and E. Chan, J. Virol. 19:13-18, 1976) and the ability to form Rev-Am-MLV pseudotypes. Data from these pseudotypes indicate that the block to Rev replication in rat cells is posttranscriptional.
摘要
网状内皮组织增殖症病毒（Rev）能在鸡和狗的细胞中复制，但不能在大鼠细胞中复制。双嗜性鼠白血病病毒（Am-MLV）能在鸡、狗和大鼠细胞中复制。通过氯霉素乙酰转移酶测定法确定，Rev长末端重复序列的转录与大鼠细胞中MLV长末端重复序列的转录没有显著差异。为了进一步确定逆转录病毒生命周期中Rev在大鼠细胞中复制受阻的步骤，我们利用了Am-MLV广泛的宿主范围（S. Rasheed、M. B. Gardner和E. Chan，《病毒学杂志》19:13 - 18，1976年）以及形成Rev-Am-MLV假型的能力。来自这些假型的数据表明，Rev在大鼠细胞中复制的受阻发生在转录后阶段。
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