Emerman M, Temin H M
Nucleic Acids Res. 1986 Dec 9;14(23):9381-96. doi: 10.1093/nar/14.23.9381.
Previously, we described "promoter suppression" in infectious retrovirus vectors with two genes and an internal promoter. Here, we examined several parameters of promoter suppression and found that the amount of suppression in an integrated retrovirus vector was dependent both on whether the vector was derived from spleen necrosis virus or murine leukemia virus and on which internal promoter was present in the vector. Murine leukemia virus vectors showed less suppression than analogous spleen necrosis virus vectors. Furthermore, the amount of suppression was not dependent on either the relative strengths of the promoters nor the distance between the promoters. Moreover, we found that in vectors in which one promoter was suppressed, there was an inverse correlation between the DNaseI sensitivity of the chromatin surrounding a promoter and the suppression of its expression.
此前，我们描述了具有两个基因和一个内部启动子的感染性逆转录病毒载体中的“启动子抑制”现象。在此，我们研究了启动子抑制的几个参数，发现整合的逆转录病毒载体中的抑制量既取决于载体是源自脾坏死病毒还是鼠白血病病毒，也取决于载体中存在哪个内部启动子。鼠白血病病毒载体的抑制作用比类似的脾坏死病毒载体小。此外，抑制量既不取决于启动子的相对强度，也不取决于启动子之间的距离。而且，我们发现，在一个启动子被抑制的载体中，启动子周围染色质的DNaseI敏感性与其表达抑制之间存在负相关。
