Mirazo Santiago, D Albora Cecilia, Quintero Gil Diana, Cabrera Karina, Ramos Natalia, Ordúz Sergio, Arbiza Juan
Sección Virologia, Facultad de Ciencias Universidad de la República, Igua 4225, 11400, Montevideo, Uruguay.
Facultad de Ciencias, Universidad Nacional de Colombia, Medellín, Colombia.
Arch Virol. 2018 Dec;163(12):3403-3407. doi: 10.1007/s00705-018-4025-1. Epub 2018 Sep 15.
Hepatitis E virus (HEV) infection involving zoonotic genotypes is a public health problem in high-income and non-endemic developing countries. Herein we report the detection of a human genotype 1 (HEV-1) strain infecting a domestic pig, which is not considered a natural reservoir of this genotype. Viral load was quantified in stool by Real-Time qPCR and sequence analyses were performed. Infectivity of the HEV-1 strain was assesed by in vitro isolation in A549 cell line. Results suggest that certain epidemiological settings might favour accidental spillover infection and thus influence the host range restriction of HEV.
戊型肝炎病毒（HEV）感染涉及人畜共患基因型，这在高收入和非流行的发展中国家是一个公共卫生问题。在此，我们报告检测到一株感染家猪的人类1型（HEV-1）毒株，而家猪并不被认为是该基因型的天然宿主。通过实时定量PCR对粪便中的病毒载量进行定量，并进行序列分析。通过在A549细胞系中进行体外分离来评估HEV-1毒株的传染性。结果表明，某些流行病学情况可能有利于意外的溢出感染，从而影响HEV的宿主范围限制。
