Department of Restorative Dentistry, Araraquara School of Dentistry, UNESP - São Paulo State University, São Paulo, Brazil.
Secretariat of the Mexican Navy, Mexico City, Mexico.
Int Endod J. 2019 Mar;52(3):385-392. doi: 10.1111/iej.13015. Epub 2018 Oct 8.
To assess the penetration of sodium hypochlorite (NaOCl) gel or NaOCl solutions with surfactants, and the effect of passive ultrasonic irrigation (PUI) on penetration into dentinal tubules.
Bovine incisor root canals were instrumented, the roots sectioned and the dentine blocks obtained were stained with crystal violet. Dentine blocks (n = 10 per group) were exposed to 3% NaOCl gel or 3% NaOCl solution for 10 and 20 min. Other dentine blocks (n = 10 per group) were exposed to Chlor-Extra (6% NaOCl + surfactant), 6% NaOCl, 2.5% NaOCl with 0.2% cetrimide and 2.5% NaOCl for 10 and 20 min. The penetration depth of irrigants into dentinal tubules was measured in micrometres by viewing the bleached crystal violet under a stereomicroscope. Additionally, bovine incisor root canals, instrumented and stained with crystal violet, were distributed into two groups (n = 10) and irrigated with 2.5% NaOCl with PUI or conventional syringe irrigation (CSI). The penetration depth of irrigants into dentinal tubules was assessed 3 and 7 mm from the apex. Statistical analysis was performed by ANOVA and Tukey tests (α = 0.05).
There was significantly greater penetration of 3% NaOCl solution into dentinal tubules compared with the gel form (P < 0.05). There was no difference (P > 0.05) between 6% NaOCl and Chlor-Extra, and between 2.5% NaOCl and 2.5% NaOCl + cetrimide. PUI significantly increased the penetration depth of NaOCl into dentinal tubules when compared with CSI (P < 0.05).
In extracted bovine incisors, NaOCl gel penetrated less into dentinal tubules than NaOCl solution. The addition of surfactants did not increase the penetration depth. The use of PUI significantly increased NaOCl penetration into dentinal tubules.
评估次氯酸钠（NaOCl）凝胶或含表面活性剂的 NaOCl 溶液的渗透情况，以及被动超声冲洗（PUI）对进入牙本质小管的影响。
牛切牙根管经过器械预备，将牙根切成段，获得牙本质块并用结晶紫染色。将牙本质块（每组 10 个）暴露于 3%的 NaOCl 凝胶或 3%的 NaOCl 溶液中 10 分钟和 20 分钟。将其他牙本质块（每组 10 个）暴露于 Chlor-Extra（6%NaOCl+表面活性剂）、6%NaOCl、含 0.2%西曲溴铵的 2.5%NaOCl 和 2.5%NaOCl 中 10 分钟和 20 分钟。通过立体显微镜观察漂白的结晶紫来测量冲洗剂进入牙本质小管的渗透深度，以微米为单位进行测量。此外，将牛切牙根管进行器械预备并结晶紫染色，分为两组（每组 10 个），分别用 PUI 或常规注射器冲洗（CSI）冲洗 2.5%的 NaOCl。评估冲洗剂进入牙本质小管的渗透深度，从根尖 3 毫米和 7 毫米处进行评估。采用方差分析和 Tukey 检验进行统计学分析（α=0.05）。
与凝胶形式相比，3%的 NaOCl 溶液进入牙本质小管的渗透深度显著更大（P<0.05）。6%的 NaOCl 与 Chlor-Extra 之间以及 2.5%的 NaOCl 与 2.5%的 NaOCl+西曲溴铵之间没有差异（P>0.05）。与 CSI 相比，PUI 显著增加了 NaOCl 进入牙本质小管的渗透深度（P<0.05）。
在提取的牛切牙中，NaOCl 凝胶进入牙本质小管的渗透深度小于 NaOCl 溶液。添加表面活性剂并没有增加渗透深度。使用 PUI 可显著增加 NaOCl 进入牙本质小管的渗透。
