Choudhury Prerana, Raghu Ramya, Shetty Ashish, Santhosh Lekha, Subhashini R, Nikhitha K L
Department of Conservative Dentistry and Endodontics, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka, India.
J Conserv Dent Endod. 2024 Jul;27(7):724-729. doi: 10.4103/JCDE.JCDE_143_24. Epub 2024 Jul 8.
The aim of the study was to compare the activity of sodium hypochlorite (NaOCl) against when used with four different irrigation protocols.
Sixty-five single-rooted mandibular premolars with closed apex were prepared till size 35/0.04. The specimens were sterilized and infected with colonies that were cultured separately. The canals were randomly divided into four experimental groups based on irrigation activation protocol, with each group having 15 specimens each - Group 1: control, Group 2: manual dynamic agitation (MDA), Group 3: passive ultrasonic irrigation (PUI), Group 4: intracanal heating (ICH), and Group 5: passive ultrasonic irrigation followed by ICH (PUI ICH). The dentinal shavings were collected and sampled before (S1) and after (S2) the different irrigation techniques were performed. The colony-forming units were counted, and the bacterial reduction was calculated for each group.
A significant reduction in the number of colonies was observed for all the experimental groups ( < 0.001). The groups with ICH of NaOCl showed a considerable reduction in bacterial colonies than other groups ( < 0.001), with Group 5 that combined ultrasonics with ICH showed the highest reduction.
ICH of NaOCl may be used as an adjunct to root canal irrigation to reduce the bacterial concentration from root canal spaces.
本研究的目的是比较次氯酸钠（NaOCl）在与四种不同冲洗方案联合使用时对[此处原文缺失具体对象]的活性。
选取65颗根尖封闭的单根下颌前磨牙，预备至35/0.04尺寸。将标本灭菌后接种分别培养的[此处原文缺失具体细菌名称]菌落。根据冲洗激活方案将根管随机分为四个实验组，每组15个标本——第1组：对照组，第2组：手动动态搅拌（MDA），第3组：被动超声冲洗（PUI），第4组：根管内加热（ICH），第5组：被动超声冲洗后进行ICH（PUI ICH）。在进行不同冲洗技术之前（S1）和之后（S2）收集牙本质碎屑并取样。计算菌落形成单位数量，并计算每组的细菌减少量。
所有实验组的[此处原文缺失具体细菌名称]菌落数量均显著减少（<0.001）。使用NaOCl进行ICH的组比其他组的细菌菌落减少量更大（<0.001），其中将超声与ICH相结合的第5组减少量最高。
NaOCl的ICH可作为根管冲洗的辅助手段，以降低根管空间中的细菌浓度。
