Gronesova Paulina, Cholujova Danka, Kozic Katarina, Korbuly Michaela, Vlcek Miroslav, Penesova Adela, Imrich Richard, Sedlak Jan, Hunakova Luba
Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Gen Physiol Biophys. 2018 Jul;37(4):443-451. doi: 10.4149/gpb_2018007.
The aim of our prospective, interventional, pre-post, single arm study was to supplement the lack of knowledge of the effect of short-term Pilates intervention on selected blood parameters of healthy women. Female volunteers were recruited for 2-weeks Pilates intervention. Blood has been collected and anthropometric parameters were measured before and after exercise period (EP). Plasma insulin, cortisol, and dehydroepiandrosterone sulphate levels, erythrocyte antioxidant activity, glutathione levels, NK cytotoxicity and plasma cytokines were analysed. We found a decrease in erythrocyte antioxidant enzymes SOD and GPx activity; GSH levels; in the pro-inflammatory chemokine MCP-1 and trend to reduction in MIP-1β, PDGF and VEGF levels in plasma. NK cell cytotoxic activity increased after Pilates EP in the percentage of specific lysis at 25:1 effector: target (E:T) ratio and the same trend was observed at all E:T ratios as well as in the amount of lytic units per 107 cells. Our findings show that Pilates exercise may improve NK cell immune response and inflammatory milieu in plasma of healthy women.
我们这项前瞻性、干预性、前后对照、单臂研究的目的是补充关于短期普拉提干预对健康女性特定血液参数影响的知识空白。招募女性志愿者进行为期两周的普拉提干预。在运动期（EP）前后采集血液并测量人体测量学参数。分析血浆胰岛素、皮质醇和硫酸脱氢表雄酮水平、红细胞抗氧化活性、谷胱甘肽水平、自然杀伤细胞（NK）细胞毒性和血浆细胞因子。我们发现红细胞抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性降低；谷胱甘肽（GSH）水平降低；促炎趋化因子单核细胞趋化蛋白-1（MCP-1）水平降低，血浆中巨噬细胞炎性蛋白-1β（MIP-1β）、血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）水平有降低趋势。在效应细胞与靶细胞比例为25:1时，普拉提运动期后NK细胞的特异性杀伤百分比细胞毒性活性增加，在所有效应细胞与靶细胞比例下以及每107个细胞的裂解单位数量上均观察到相同趋势。我们的研究结果表明，普拉提运动可能改善健康女性血浆中的NK细胞免疫反应和炎症环境。
