Tufano M A, Sommese L, Capasso C, Folgore A, Scafa F, Galdiero E
Microbiologica. 1986 Oct;9(4):431-42.
The outer membrane of Gram-negative bacteria contains some major proteins, called porins, with particular chemical and physical characteristics which reflect some specialized functions peculiar to the outer membrane. In recent years the biological properties of Salmonella typhimurium SH5014 porins have been the focus of our experimental studies. The aim of the present research was to make a comparative investigation of the biological activities of porins extracted from other microorganisms classified in the Enterobacteriaceae, together with a taxonomically different one, Eikenella corrodens frequently isolated from gum pockets of patients with periodontal disease. Porins from E. coli K12, Proteus mirabilis and Eikenella corrodens were extracted, purified and separated from LPS by means of phenol extraction. The purity of preparation was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis in slabs. We studied the interactions of these proteins with some cellular and humoral systems within the host. Mouse peritoneal macrophages in the presence of these proteins show morphological and functional modifications, depending upon the type of porins and the concentrations used. The ability of porins to interact with the complement system was investigated in vitro. A pool of sera from 20 blood donors served as a source of human complement. The results obtained with porins of various sources were shown comparatively.
革兰氏阴性菌的外膜含有一些主要蛋白质，称为孔蛋白，其具有特定的化学和物理特性，反映了外膜特有的一些专门功能。近年来，鼠伤寒沙门氏菌SH5014孔蛋白的生物学特性一直是我们实验研究的重点。本研究的目的是对从肠杆菌科分类的其他微生物以及从牙周病患者牙龈袋中经常分离出的分类不同的腐蚀艾肯菌中提取的孔蛋白的生物活性进行比较研究。通过酚提取法从大肠杆菌K12、奇异变形杆菌和腐蚀艾肯菌中提取、纯化孔蛋白并将其与脂多糖分离。通过十二烷基硫酸钠聚丙烯酰胺平板凝胶电泳检查制备物的纯度。我们研究了这些蛋白质与宿主体内一些细胞和体液系统的相互作用。在这些蛋白质存在的情况下，小鼠腹腔巨噬细胞会出现形态和功能改变，这取决于孔蛋白的类型和所用浓度。在体外研究了孔蛋白与补体系统相互作用的能力。来自20名献血者的血清池用作人类补体的来源。比较展示了用各种来源的孔蛋白获得的结果。
