Ogawa Y, Sagata N, Tsuzuku-Kawamura J, Onuma M, Izawa H, Ikawa Y
Microbiol Immunol. 1986;30(7):697-701. doi: 10.1111/j.1348-0421.1986.tb02995.x.
Using various portions of a molecularly cloned bovine leukemia virus (BLV) DNA as probes, the possible integration of a BLV genome or a BLV-related sequence into the chromosomal DNA of sporadic bovine leukosis (SBL) tumor cells was investigated by Southern blotting analysis. Under stringent as well as nonstringent conditions of hybridization, neither BLV nor BLV-related sequence specific to SBL DNAs was detected in any SBL tumor examined. These results provide conclusive evidence for lack of the relation of BLV or a BLV-related agent to SBL.
利用分子克隆的牛白血病病毒（BLV）DNA的不同片段作为探针，通过Southern印迹分析研究了BLV基因组或BLV相关序列在散发性牛白血病（SBL）肿瘤细胞染色体DNA中的可能整合情况。在严格及非严格杂交条件下，在所检测的任何SBL肿瘤中均未检测到SBL DNA特有的BLV或BLV相关序列。这些结果为BLV或BLV相关因子与SBL缺乏关联提供了确凿证据。
