牛白血病病毒：一种外源性RNA致癌病毒。
Bovine leukemia virus: an exogenous RNA oncogenic virus.
作者信息
Kettmann R, Portetelle D, Mammerickx M, Cleuter Y, Dekegel D, Galoux M, Ghysdael J, Burny A, Chantrenne H
出版信息
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1014-8. doi: 10.1073/pnas.73.4.1014.
Short-term cultures of bovine leukemic lymphocytes release virus particles with biochemical properties of RNA oncogenic viruses. These particles, tentatively called bovine leukemia virus (BLV), have a high molecular weight RNA-reverse transcriptase complex and a density of 1.155 g/ml in sucrose solutions. Molecular hybridizations between BLV/[3H]cDNA and several viral RNAs show that BLV is not related to Mason-Pfizer monkey virus, simian sarcoma associated virus, feline leukemia virus, or avian myeloblastosis virus. These results were confirmed by hybridization between BLV 70S RNA and [3H]cDNA synthesized in the various viruses tested. The high preference of BLV reverse transciptase for Mg++ as the divalent cation suggests that BLV might be an atypical mammalian leukemogenic "type C" virus. DNA-DNA hybridization studies using BLV [3H]cDNA as a probe strongly suggest that the DNA of bovine leukemic cells contains viral sequences that cannot be detected in normal bovine DNA.
牛白血病淋巴细胞的短期培养物释放出具有RNA致癌病毒生化特性的病毒颗粒。这些颗粒，暂称为牛白血病病毒（BLV），具有高分子量的RNA逆转录酶复合物，在蔗糖溶液中的密度为1.155 g/ml。BLV/[3H]cDNA与几种病毒RNA之间的分子杂交表明，BLV与梅森- Pfizer猴病毒、猿猴肉瘤相关病毒、猫白血病病毒或禽成髓细胞瘤病毒无关。在测试的各种病毒中合成的BLV 70S RNA与[3H]cDNA之间的杂交证实了这些结果。BLV逆转录酶对Mg++作为二价阳离子的高度偏好表明，BLV可能是一种非典型的哺乳动物致白血病“C型”病毒。使用BLV [3H]cDNA作为探针的DNA-DNA杂交研究强烈表明，牛白血病细胞的DNA含有在正常牛DNA中无法检测到的病毒序列。
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