Duan Rongquan, Barbieri Davide, de Groot Florence, de Bruijn Joost D, Yuan Huipin
Biomaterial Science and Technology, MIRA, University of Twente, 7522 NB Enschede, The Netherlands.
Kuros Biosciences BV, 3723 MB Bilthoven, The Netherlands.
ACS Biomater Sci Eng. 2018 Sep 10;4(9):3347-3355. doi: 10.1021/acsbiomaterials.8b00630. Epub 2018 Jul 26.
Tricalcium phosphate (TCP) ceramics are used as bone void fillers because of their bioactivity and resorbability, while their performance in bone regeneration and material resorption vary with their physical properties (e.g., the dimension of the crystal grain). Herein, three TCP ceramic bone substitutes (TCP-S, TCP-M, and TCP-L) with gradient crystal grain size (0.77 ± 0.21 μm for TCP-S, 1.21 ± 0.35 μm for TCP-M and 4.87 ± 1.90 μm for TCP-L), were evaluated in a well-established rabbit lateral condylar defect model (validated with sham) with respect to bone formation and material resorption up to 26 weeks. Surface structure-dependent bone regeneration was clearly shown after 4 weeks implantation with TCP-S having most mineralized bone (20.2 ± 3.4%), followed by TCP-M (14.0 ± 3.5%), sham (8.1 ± 4.2%), and TCP-L (6.6 ± 2.6%). Afterward, the amount of mineralized bone was similar in all the three groups, but bone marrow and material resorption varied. After 26 weeks, TCP-S induced most bone tissue formation (mineralized bone + bone marrow) (61.6 ± 7.8%) and underwent most material resorption (80.1 ± 9.0%), followed by TCP-M (42.9 ± 5.2% and 61.4 ± 8.0% respectively), TCP-L (28.3 ± 5.5% and 45.6 ± 9.7% respectively), and sham (25.7 ± 4.2%). Given the fact that the three ceramics are chemically identical, the results indicate that the surface structure (especially, the crystal grain size) of TCP ceramics can greatly tune their bone regeneration potential and the material resorption in rabbit condyle defect model, with the submicron surface structured TCP ceramic performing the best.
磷酸三钙（TCP）陶瓷因其生物活性和可吸收性而被用作骨缺损填充材料，但其在骨再生和材料吸收方面的性能会因其物理性质（如晶粒尺寸）而有所不同。在此，三种具有梯度晶粒尺寸的TCP陶瓷骨替代物（TCP-S，TCP-M和TCP-L，其中TCP-S的晶粒尺寸为0.77±0.21μm，TCP-M为1.21±0.35μm，TCP-L为4.87±1.90μm），在一个成熟的兔外侧髁突缺损模型（通过假手术验证）中，对其长达26周的骨形成和材料吸收情况进行了评估。植入4周后，表面结构依赖性骨再生明显，TCP-S的矿化骨最多（20.2±3.4%），其次是TCP-M（14.0±3.5%）、假手术组（8.1±4.2%）和TCP-L（6.6±2.6%）。此后，所有三组中的矿化骨量相似，但骨髓和材料吸收情况有所不同。26周后，TCP-S诱导的骨组织形成最多（矿化骨+骨髓）（61.6±7.8%），材料吸收也最多（80.1±9.0%），其次是TCP-M（分别为42.9±5.2%和61.4±8.0%）、TCP-L（分别为28.3±5.5%和45.6±9.7%）以及假手术组（25.7±4.2%）。鉴于这三种陶瓷在化学上是相同的，结果表明TCP陶瓷的表面结构（特别是晶粒尺寸）可以极大地调节其在兔髁突缺损模型中的骨再生潜力和材料吸收情况，其中具有亚微米表面结构的TCP陶瓷表现最佳。
