Hasemann Georg, Betke Ulf, Krüger Manja, Walles Heike, Scheffler Michael
Institute of Materials and Joining Technology, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
Institute of Chemistry-Core Facility Tissue Engineering, Otto-von-Guericke-University Magdeburg, Pfaelzerstr. 2, 39106 Magdeburg, Germany.
Materials (Basel). 2021 May 25;14(11):2813. doi: 10.3390/ma14112813.
Ceramics are widely used as implant materials; however, they are brittle and may emit particles when used in these applications. To overcome this disadvantage, alumina foams, which represent a 3D cellular structure comparable to that of human trabecular bone structures, were sputter coated with platinum, tantalum or titanium and modified with fibronectin or collagen type I, components of the extracellular matrix (ECM). To proof the cell material interaction, the unmodified and modified materials were cultured with (a) mesenchymal stem cells being a perfect indicator for biocompatibility and releasing important cytokines of the stem cell niche and (b) with fibroblasts characterized as mediators of inflammation and therefore an important cellular component of the foreign body reaction and inflammation after implantation. To optimize and compare the influence of metal surfaces on cellular behavior, planar glass substrates have been used. Identified biocompatible metal surface of platinum, titanium and tantalum were sputtered on ceramic foams modified with the above-mentioned ECM components to investigate cellular behavior in a 3D environment. The cellular alumina support was characterized with respect to its cellular/porous structure and niche accessibility and coating thickness of the refractory metals; the average cell size was 2.3 mm, the average size of the cell windows was 1.8 mm, and the total foam porosity was 91.4%. The Pt, Ti and Ta coatings were completely dense covering the entire alumina foam surface. The metals titanium and tantalum were colonized very well by the stem cells without a coating of ECM components, whereas the fibroblasts preferred components of the ECM on the alumina foam surface.
陶瓷被广泛用作植入材料；然而，它们很脆，在这些应用中使用时可能会释放颗粒。为了克服这一缺点，具有与人类小梁骨结构相当的三维多孔结构的氧化铝泡沫，被溅射涂覆铂、钽或钛，并用细胞外基质（ECM）的成分纤连蛋白或I型胶原进行修饰。为了验证细胞与材料的相互作用，将未修饰和修饰后的材料分别与（a）间充质干细胞一起培养，间充质干细胞是生物相容性的完美指标，可释放干细胞微环境的重要细胞因子，以及（b）成纤维细胞一起培养，成纤维细胞被认为是炎症介质，因此是植入后异物反应和炎症的重要细胞成分。为了优化和比较金属表面对细胞行为的影响，使用了平面玻璃基板。将已确定具有生物相容性的铂、钛和钽金属表面溅射在经上述ECM成分修饰的陶瓷泡沫上，以研究三维环境中的细胞行为。对多孔氧化铝载体的细胞/多孔结构、微环境可达性和难熔金属的涂层厚度进行了表征；平均孔尺寸为2.3mm，孔窗平均尺寸为1.8mm，泡沫总孔隙率为91.4%。铂、钛和钽涂层完全致密，覆盖了整个氧化铝泡沫表面。在没有ECM成分涂层的情况下，钛和钽金属被干细胞很好地定植，而成纤维细胞更喜欢氧化铝泡沫表面的ECM成分。
