Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584, CT, Utrecht, The Netherlands.
US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA.
Biotechnol J. 2019 Apr;14(4):e1800185. doi: 10.1002/biot.201800185. Epub 2018 Oct 8.
As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications. The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS). Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH. Substrate adaptation is also observed for carbon catabolism. Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies. The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.
作为食草动物粪便的晚期殖民者，Podospora anserina 进化出了一种酶机制来降解植物生物质中更顽固的部分，这表明它在生物技术应用方面有很大的潜力。作者研究了它在两种工业饲料——大豆壳（SBH）和玉米秸秆（CS）上生长时的转录组。最初，CS 和 SBH 分别导致半纤维素酶和淀粉酶基因的表达，而在稍后的时间点，诱导了更多种类的基因，特别是对于 SBH。碳分解代谢也观察到了底物适应性。总的来说，SBH 导致了更多表达基因的多样性，这与之前的蛋白质组学研究结果一致。这些结果不仅深入了解了 P. anserina 对底物组成的转录组适应，还指出了在工业水平上提高植物生物质糖化的策略。
