Tn7转座：一个多基因过程。一种调控基因产物的鉴定。

Tn7 transposition: a multigene process. Identification of a regulatory gene product.

作者信息

Smith G M, Jones P

出版信息

Nucleic Acids Res. 1986 Oct 24;14(20):7915-27. doi: 10.1093/nar/14.20.7915.

DOI:10.1093/nar/14.20.7915

PMID:3022239

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311824/

Abstract

Tn7 transposition is abolished by the deletion of a 2.2kb HindIII fragment from the central region of the transposon. Transposition is restored when the fragment is present in trans. When this fragment is present in trans with wild-type Tn7, transposition frequencies are stimulated 10-100-fold. The DNA sequence of this fragment has been determined and found to contain one long open reading frame coding for a protein of molecular weight 61 187. We have visualised this protein using a DNA-directed prokaryotic transcription-translation system. This gene may fill a regulatory role in the mechanism of Tn7 transposition. When present in trans the 2.2kb HindIII fragment alleviates a transcriptional block of promoter activity detected in Tn7.

摘要

从转座子中央区域缺失一个2.2kb的HindIII片段会使Tn7转座作用消失。当该片段以反式存在时，转座作用得以恢复。当此片段与野生型Tn7以反式存在时，转座频率会提高10至100倍。已确定该片段的DNA序列，发现其包含一个长的开放阅读框，编码一种分子量为61187的蛋白质。我们使用DNA指导的原核转录-翻译系统使这种蛋白质可视化。该基因可能在Tn7转座机制中起调节作用。当以反式存在时，2.2kb的HindIII片段可缓解在Tn7中检测到的启动子活性的转录阻滞。