Gosti-Testu F, Norris V, Brevet J
Plasmid. 1983 Jul;10(1):96-9. doi: 10.1016/0147-619x(83)90061-6.
Tn7, a transposon of 14 kb, encodes resistance to trimethoprim (Tp) and streptomycin (Sm). A cleavage site map of this transposon for twenty-two different restriction enzymes as determined by comparison of restriction enzyme cleavage patterns of the plasmids ColE1 and ColE1::Tn7 is presented. The precise localization of these sites was facilitated by the use of two deletion derivatives of ColE1::Tn7: pGB2 and ColE1::Tn7 delta 6, and by the use of pOB14 and pOB15 which contain a part of Tn7 cloned into the plasmid pBR322. This map should aid in the study of the structural and genetic organization of this transposon.
Tn7是一个14千碱基对的转座子，编码对甲氧苄啶（Tp）和链霉素（Sm）的抗性。通过比较质粒ColE1和ColE1::Tn7的限制酶切割模式，给出了该转座子针对22种不同限制酶的切割位点图谱。通过使用ColE1::Tn7的两种缺失衍生物：pGB2和ColE1::Tn7 delta 6，以及使用含有克隆到质粒pBR322中的Tn7一部分的pOB14和pOB15，促进了这些位点的精确定位。该图谱应有助于对这个转座子的结构和遗传组织进行研究。
