DeFeo-Jones D, Scolnick E M, Koller R, Dhar R
Nature. 1983;306(5944):707-9. doi: 10.1038/306707a0.
The oncogenes of Harvey and Kirsten murine sarcoma viruses (v-rasH and v-rasK) and their cellular homologues (c-rasH and c-rasK) constitute two members of the ras gene family. Each functional member of the ras gene family encodes a 21,000 molecular weight protein (p21ras). ras genes have been detected in a wide variety of vertebrate species, including Xenopus laevis (R. E. Steele, personal communication), and in Drosophila melanogaster. We report here the detection of ras-related genes in the yeast Saccharomyces cerevisiae, and the isolation of two ras-related molecular clones, c-rassc-1 and c-rassc-2, from the DNA of Saccharomyces. Both c-rassc-1 and c-rassc-2 hybridize specifically to probes prepared from mammalian ras DNA. Sequencing of c-rassc-1 reveals extensive amino acid homology between the protein encoded by c-rassc-1 and the p21 encoded by c-rasH. Our studies suggest that these clones can be used to elucidate the normal cellular functions of ras-related genes in this relatively simple eukaryotic organism.
哈维氏和克里斯滕氏鼠肉瘤病毒的癌基因（v-rasH和v-rasK）及其细胞同源物（c-rasH和c-rasK）构成了ras基因家族的两个成员。ras基因家族的每个功能成员都编码一种分子量为21,000的蛋白质（p21ras）。ras基因已在多种脊椎动物物种中被检测到，包括非洲爪蟾（R.E.斯蒂尔，个人交流），以及在黑腹果蝇中也有发现。我们在此报告在酿酒酵母中检测到ras相关基因，并从酿酒酵母的DNA中分离出两个ras相关分子克隆，即c-rassc-1和c-rassc-2。c-rassc-1和c-rassc-2都能与由哺乳动物ras DNA制备的探针特异性杂交。对c-rassc-1的测序揭示了c-rassc-1编码的蛋白质与c-rasH编码的p21之间存在广泛的氨基酸同源性。我们的研究表明，这些克隆可用于阐明在这种相对简单的真核生物中ras相关基因的正常细胞功能。
