基因表达调控因子中效应特异性的改变：TOL 质粒 xylS 突变体及其在工程改造中用于拓展细菌降解芳烃范围的应用。
Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria.
作者信息
Ramos J L, Stolz A, Reineke W, Timmis K N
出版信息
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8467-71. doi: 10.1073/pnas.83.22.8467.
Abstract
Stimulation of transcription from positively regulated promoters involves regulatory proteins that have been activated, generally as a consequence of binding low molecular weight effector molecules. To define essential structural features of effectors for one positively acting gene regulator, the xylS-encoded protein, which activates the TOL plasmid meta-cleavage pathway operon promoters, effector activities of a wide range of benzoate derivatives have been systematically analyzed, and mutant xylS-encoded proteins exhibiting altered effector specificities have been generated and characterized. Cloned mutant xylS genes were trans dominant in partial diploids containing the wild-type xylS allele and could therefore be used to effect expansion of the range of aromatic compounds completely or partially degraded by Pseudomonas bacteria. The method developed to isolate mutant xylS-encoded proteins has general applicability and could in principle be used to isolate gene regulator specificity mutants of any inducible regulatory system.
摘要
对正调控启动子转录的刺激涉及通常因结合低分子量效应分子而被激活的调控蛋白。为了确定一种正向作用的基因调控因子（即木糖S编码的蛋白，它激活TOL质粒间位裂解途径操纵子启动子）效应物的基本结构特征，已系统分析了多种苯甲酸酯衍生物的效应物活性，并产生和表征了表现出改变的效应物特异性的木糖S编码的突变蛋白。克隆的突变木糖S基因在含有野生型木糖S等位基因的部分二倍体中具有反式显性，因此可用于扩大假单胞菌属细菌完全或部分降解的芳香化合物范围。所开发的分离木糖S编码的突变蛋白的方法具有普遍适用性，原则上可用于分离任何诱导型调控系统的基因调控因子特异性突变体。
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Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria.基因表达调控因子中效应特异性的改变：TOL 质粒 xylS 突变体及其在工程改造中用于拓展细菌降解芳烃范围的应用。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8467-71. doi: 10.1073/pnas.83.22.8467.
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