TOL 质粒 xylS 基因的分子克隆：xylS 对 xylDEGF 操纵子正向调控的证据
Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.
作者信息
Inouye S, Nakazawa A, Nakazawa T
出版信息
J Bacteriol. 1981 Nov;148(2):413-8. doi: 10.1128/jb.148.2.413-418.1981.
Abstract
The xylDEGF operon and the regulatory gene xylS of the TOL plasmid found in Pseudomonas putida mt-2 were cloned onto Escherichia coli vector plasmids. A 9.5-kilobase fragment, derived from the TOL segment of pTN2 deoxyribonucleic acid, carried the xyl genes D, E, G, and F, which encode toluate oxygenase, catechol 2,3-oxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, and 2-hydroxymuconic semialdehyde hydrolase, respectively. The enzymes were noninducible unless a 3-kilobase PstI fragment, derived also from the TOL segment, was provided in either cis or trans. The PstI fragment appeared to contain the regulatory gene xylS, which produced a positive regulator. The regulator was activated by m-toluate or benzoate, but not by m-xylene or m-methylbenzyl alcohol. the map positions of xylG and xylF were also determined.
摘要
恶臭假单胞菌mt-2中发现的TOL质粒的xylDEGF操纵子和调控基因xylS被克隆到大肠杆菌载体质粒上。一个源自pTN2脱氧核糖核酸TOL片段的9.5千碱基片段携带了xyl基因D、E、G和F，它们分别编码甲苯酸加氧酶、儿茶酚2,3-加氧酶、2-羟基粘康酸半醛脱氢酶和2-羟基粘康酸半醛水解酶。这些酶是不可诱导的，除非同样源自TOL片段的一个3千碱基的PstI片段以顺式或反式提供。PstI片段似乎包含调控基因xylS，它产生一种正调控因子。该调控因子被间甲苯酸或苯甲酸激活，但不被间二甲苯或间甲基苄醇激活。还确定了xylG和xylF的图谱位置。
相似文献
1
Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.TOL 质粒 xylS 基因的分子克隆：xylS 对 xylDEGF 操纵子正向调控的证据
J Bacteriol. 1981 Nov;148(2):413-8. doi: 10.1128/jb.148.2.413-418.1981.
2
Molecular cloning of regulatory gene xylR and operator-promoter regions of the xylABC and xylDEGF operons of the TOL plasmid.TOL质粒调控基因xylR以及xylABC和xylDEGF操纵子的操纵子-启动子区域的分子克隆
J Bacteriol. 1983 Sep;155(3):1192-9. doi: 10.1128/jb.155.3.1192-1199.1983.
3
Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53.编码TOL质粒pWW0和pWW53中代谢途径酶的基因的进化保守性。
J Bacteriol. 1985 Nov;164(2):887-95. doi: 10.1128/jb.164.2.887-895.1985.
4
Characterization of Pseudomonas putida mutants unable to catabolize benzoate: cloning and characterization of Pseudomonas genes involved in benzoate catabolism and isolation of a chromosomal DNA fragment able to substitute for xylS in activation of the TOL lower-pathway promoter.不能分解代谢苯甲酸的恶臭假单胞菌突变体的特性：参与苯甲酸分解代谢的假单胞菌基因的克隆与特性分析以及能够在激活TOL下游途径启动子中替代xylS的染色体DNA片段的分离
J Bacteriol. 1992 Aug;174(15):4986-96. doi: 10.1128/jb.174.15.4986-4996.1992.
5
Expression of the regulatory gene xylS on the TOL plasmid is positively controlled by the xylR gene product.TOL质粒上调控基因xylS的表达受xylR基因产物的正调控。
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5182-6. doi: 10.1073/pnas.84.15.5182.
6
Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.恶臭假单胞菌TOL质粒pWWO的分子与功能分析及整个调控型芳香环间位裂解途径基因的克隆
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458-62. doi: 10.1073/pnas.78.12.7458.
7
Transposon mutagenesis analysis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2.恶臭假单胞菌mt-2的TOL质粒间位裂解途径操纵子基因的转座子诱变分析
J Bacteriol. 1984 Oct;160(1):251-5. doi: 10.1128/jb.160.1.251-255.1984.
8
The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes.TOL降解性质粒pWW0的间位裂解操纵子由13个基因组成。
Mol Gen Genet. 1990 Mar;221(1):113-20. doi: 10.1007/BF00280375.
9
Overproduction of the xylS gene product and activation of the xylDLEGF operon on the TOL plasmid.木糖S基因产物的过量产生以及TOL质粒上木糖DLEGF操纵子的激活。
J Bacteriol. 1987 Aug;169(8):3587-92. doi: 10.1128/jb.169.8.3587-3592.1987.
10
Nucleotide sequence of the regulatory gene xylS on the Pseudomonas putida TOL plasmid and identification of the protein product.恶臭假单胞菌TOL质粒上调控基因xylS的核苷酸序列及蛋白产物的鉴定。
Gene. 1986;44(2-3):235-42. doi: 10.1016/0378-1119(86)90187-3.
引用本文的文献
1
AND Logic Based on Suppressor tRNAs Enables Stringent Control of Sliding Base Editors in .基于抑制性tRNA的与逻辑实现了对滑动碱基编辑器的严格控制。
ACS Synth Biol. 2024 Dec 20;13(12):4191-4201. doi: 10.1021/acssynbio.4c00640. Epub 2024 Dec 11.
2
Acinetobacter sp. DW-1 immobilized on polyhedron hollow polypropylene balls and analysis of transcriptome and proteome of the bacterium during phenol biodegradation process.不动杆菌 DW-1 固定在多面体中空聚丙烯球上，以及在苯酚生物降解过程中细菌的转录组和蛋白质组分析。
Sci Rep. 2017 Jul 7;7(1):4863. doi: 10.1038/s41598-017-04187-6.
3
Transcription factor-based biosensors enlightened by the analyte.由分析物启发的基于转录因子的生物传感器。
Front Microbiol. 2015 Jul 1;6:648. doi: 10.3389/fmicb.2015.00648. eCollection 2015.
4
Gene expression in Pseudomonas.假单胞菌中的基因表达。
World J Microbiol Biotechnol. 1993 Jul;9(4):433-43. doi: 10.1007/BF00328031.
5
Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression.对PM启动子进行随机诱变是提高重组基因表达的一种有效策略。
Appl Environ Microbiol. 2009 Apr;75(7):2002-11. doi: 10.1128/AEM.02315-08. Epub 2009 Feb 5.
6
Bacterial transcriptional regulators for degradation pathways of aromatic compounds.用于芳香族化合物降解途径的细菌转录调节因子。
Microbiol Mol Biol Rev. 2004 Sep;68(3):474-500, table of contents. doi: 10.1128/MMBR.68.3.474-500.2004.
7
BenR, a XylS homologue, regulates three different pathways of aromatic acid degradation in Pseudomonas putida.BenR是XylS的同源物，可调控恶臭假单胞菌中三种不同的芳香酸降解途径。
J Bacteriol. 2000 Nov;182(22):6339-46. doi: 10.1128/JB.182.22.6339-6346.2000.
8
Characterization of cell lysis in Pseudomonas putida induced upon expression of heterologous killing genes.恶臭假单胞菌中异源杀伤基因表达诱导的细胞裂解特性分析
Appl Environ Microbiol. 1998 Dec;64(12):4904-11. doi: 10.1128/AEM.64.12.4904-4911.1998.
9
Contribution of indole-3-acetic acid production to the epiphytic fitness of erwinia herbicola.吲哚-3-乙酸的产生对草生欧文氏菌附生适应性的贡献。
Appl Environ Microbiol. 1998 Sep;64(9):3256-63. doi: 10.1128/AEM.64.9.3256-3263.1998.
10
Activation and repression of transcription at the double tandem divergent promoters for the xylR and xylS genes of the TOL plasmid of Pseudomonas putida.恶臭假单胞菌TOL质粒xylR和xylS基因双串联反向启动子转录的激活与抑制
J Bacteriol. 1998 Jun;180(11):2889-94. doi: 10.1128/JB.180.11.2889-2894.1998.
本文引用的文献
1
Molecular cloning of TOL genes xylB and xylE in Escherichia coli.大肠杆菌中TOL基因xylB和xylE的分子克隆
J Bacteriol. 1981 Mar;145(3):1137-43. doi: 10.1128/jb.145.3.1137-1143.1981.
2
Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.构建恶臭假单胞菌mt-2的TOL质粒（pWWO）编码的降解途径的部分二倍体：xyIR基因正向调控性质的证据。
Mol Gen Genet. 1980 Jan;177(2):321-8. doi: 10.1007/BF00267445.
3
Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants.RP4-TOL 质粒重组体的物理和功能图谱：插入和缺失突变体分析
J Bacteriol. 1980 Oct;144(1):222-31. doi: 10.1128/jb.144.1.222-231.1980.
4
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.大肠杆菌L-阿拉伯糖操纵子：调节蛋白的结合位点及正负调控机制
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3346-50. doi: 10.1073/pnas.77.6.3346.
5
Benzoate metabolism in Pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways.恶臭假单胞菌（阿维拉）mt-2中的苯甲酸代谢：两条苯甲酸途径的证明
J Bacteriol. 1973 Jul;115(1):262-7. doi: 10.1128/jb.115.1.262-267.1973.
6
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
7
Regulation: positive control.调控：阳性对照。
Annu Rev Genet. 1974;8:219-42. doi: 10.1146/annurev.ge.08.120174.001251.
8
Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.恶臭假单胞菌（Pseudomonas (putida (arvilla) mt-2）对甲苯和二甲苯的代谢：TOL 质粒新功能的证据
J Bacteriol. 1975 Oct;124(1):7-13. doi: 10.1128/jb.124.1.7-13.1975.
9
Regulation of the degradative pathway enzymes coded for by the TOL plasmid (pWWO) from Pseudomonas putida mt-2.恶臭假单胞菌mt-2的TOL质粒（pWWO）编码的降解途径酶的调控
J Bacteriol. 1978 Jun;134(3):757-64. doi: 10.1128/jb.134.3.757-764.1978.
10
Isolation of TOL and RP4 recombinants by integrative suppression.通过整合抑制分离TOL和RP4重组体。
J Bacteriol. 1978 Apr;134(1):270-7. doi: 10.1128/jb.134.1.270-277.1978.
Zotero 插件