Allen J P, Feher G, Yeates T O, Rees D C, Deisenhofer J, Michel H, Huber R
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8589-93. doi: 10.1073/pnas.83.22.8589.
Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P2(1)2(1)2(1), have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.
对来自球形红假单胞菌、空间群为P2(1)2(1)2(1)的反应中心（RC）晶体进行了X射线衍射研究。采用帕特森搜索（分子置换）技术分析数据，以嗜绿红假单胞菌反应中心的结构作为模型系统。已获得球形红假单胞菌反应中心的初步电子密度图。比较球形红假单胞菌与嗜绿红假单胞菌反应中心的结构，发现以下保守特征：L和M亚基各有五个跨膜螺旋，H亚基有一个跨膜螺旋，一个二重对称轴，以及辅因子的相似位置和取向。与嗜绿红假单胞菌的反应中心不同，球形红假单胞菌的反应中心保留了两个醌。次级醌位于与初级醌通过二重对称轴相关的位置附近。
