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穿过含有细菌反应中心的平面双层膜的光电流。单电子周转条件下的响应。

Photoelectric currents across planar bilayer membranes containing bacterial reaction centers. Response under conditions of single electron turnover.

作者信息

Packham N K, Dutton P L, Mueller P

出版信息

Biophys J. 1982 Feb;37(2):465-73. doi: 10.1016/S0006-3495(82)84693-6.

Abstract

Light-induced electric current and potential responses have been measured across planar phospholipid membranes containing reaction centers from the photosynthetic bacterium Rhodopseudomonas sphaeroides. Under conditions in which the reaction centers are restricted to a single electron turnover, the responses can be correlated with the light-induced electron transfer reactions associated with the reaction center. The results indicate that electron transfer from the bacteriochlorophyll dimer to the primary ubiquinone molecule, and from ferrocytochrome c to the oxidized dimer occur in series across the planar membrane. Electron transfer from the primary to secondary ubiquinone molecule is not electrogenic.

摘要

已经测量了穿过含有球形红假单胞菌光合细菌反应中心的平面磷脂膜的光诱导电流和电位响应。在反应中心限于单电子周转的条件下,这些响应可以与与反应中心相关的光诱导电子转移反应相关联。结果表明,从细菌叶绿素二聚体到初级泛醌分子以及从亚铁细胞色素c到氧化二聚体的电子转移是跨平面膜串联发生的。从初级到次级泛醌分子的电子转移不产生电效应。

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Hexane-solubilised reaction centre proteolipid complexes of Rhodopseudomonas sphaeroides.
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