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穿过含有细菌反应中心的平面双层膜的光电流。单电子周转条件下的响应。

Photoelectric currents across planar bilayer membranes containing bacterial reaction centers. Response under conditions of single electron turnover.

作者信息

Packham N K, Dutton P L, Mueller P

出版信息

Biophys J. 1982 Feb;37(2):465-73. doi: 10.1016/S0006-3495(82)84693-6.

DOI:10.1016/S0006-3495(82)84693-6
PMID:6277403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1328829/
Abstract

Light-induced electric current and potential responses have been measured across planar phospholipid membranes containing reaction centers from the photosynthetic bacterium Rhodopseudomonas sphaeroides. Under conditions in which the reaction centers are restricted to a single electron turnover, the responses can be correlated with the light-induced electron transfer reactions associated with the reaction center. The results indicate that electron transfer from the bacteriochlorophyll dimer to the primary ubiquinone molecule, and from ferrocytochrome c to the oxidized dimer occur in series across the planar membrane. Electron transfer from the primary to secondary ubiquinone molecule is not electrogenic.

摘要

已经测量了穿过含有球形红假单胞菌光合细菌反应中心的平面磷脂膜的光诱导电流和电位响应。在反应中心限于单电子周转的条件下,这些响应可以与与反应中心相关的光诱导电子转移反应相关联。结果表明,从细菌叶绿素二聚体到初级泛醌分子以及从亚铁细胞色素c到氧化二聚体的电子转移是跨平面膜串联发生的。从初级到次级泛醌分子的电子转移不产生电效应。

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2
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本文引用的文献

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Functional reconstitution of photosynthetic reaction centers in planar lipid bilayers.光合反应中心在平面脂质双分子层中的功能重建。
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CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.源自鸡蛋磷脂的色谱纯卵磷脂
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PROTEOLIPIDS. I. FORMATION OF PHOSPHOLIPID-CYTOCHROME C COMPLEXES.蛋白脂质。一、磷脂 - 细胞色素C复合物的形成。
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The high energy state in chromatophores from Rhodopseudomonas spheroides.球形红假单胞菌色素细胞中的高能态。
FEBS Lett. 1969 Aug;4(3):185-189. doi: 10.1016/0014-5793(69)80230-9.
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Generation of membrane potential during photosynthetic electron flow in chromatophores from Rhodopseudomonas capsulata.荚膜红假单胞菌载色体光合电子流过程中膜电位的产生
Biochim Biophys Acta. 1980 Aug 5;592(1):130-42. doi: 10.1016/0005-2728(80)90120-6.
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Hexane-solubilised reaction centre proteolipid complexes of Rhodopseudomonas sphaeroides.
Biochim Biophys Acta. 1981 May 13;635(3):585-601. doi: 10.1016/0005-2728(81)90116-x.
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Reconstitution of photochemically active reaction centers in planar phospholipid membranes. Light-induced electrical currents under voltage-clamped conditions.平面磷脂膜中光化学活性反应中心的重建。电压钳制条件下的光诱导电流。
FEBS Lett. 1980 Jan 28;110(1):101-6. doi: 10.1016/0014-5793(80)80033-0.
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Reaction center--phospholipid complex in organic solvents: formation and properties.有机溶剂中的反应中心 - 磷脂复合物:形成与性质
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The kinetics of light induced carotenoid changes in Rhodopseudomonas spheroides and their relation to electrical field generation across the chromatophore membrane.球形红假单胞菌中光诱导类胡萝卜素变化的动力学及其与跨色素细胞膜产生电场的关系。
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In Chromatium, a single photochemical reaction center oxidizes both cytochrome C552 and cytochrome C555.在嗜硫红假单胞菌中,单一的光化学反应中心会氧化细胞色素C552和细胞色素C555。
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