1 Department of Biomedical Engineering, College of Mechanics, Taiyuan University of Technology, Taiyuan, PR China.
2 Institute of Applied Mechanics & Biomedical Engineering, Taiyuan University of Technology, Taiyuan, PR China.
J Biomater Appl. 2018 Sep;33(3):402-409. doi: 10.1177/0885328218797545.
In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering. The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated. The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure. The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds. Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively. The pore size and porosity of these scaffolds were suitable for bone regeneration. Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells. The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation. The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
在这项研究中，通过将β-环糊精与六亚甲基二异氰酸酯和羟基磷灰石原位聚合，首次成功制备了含有羟基磷灰石和β-环糊精基聚氨酯的新型多孔支架，用于骨组织工程。研究了多孔支架的物理化学和机械性能以及细胞相容性。结果表明，用羟基磷灰石复合材料增强的聚氨酯具有松质骨样多孔结构。支架的机械强度随支架中羟基磷灰石含量的增加而增加。合成的支架（PU1、PUHA1、PU2 和 PUHA2）的压缩强度值分别为 0.87±0.24 MPa、1.81±0.10 MPa、6.16±0.89 MPa 和 12.95±2.05 MPa。这些支架的孔径和孔隙率适合骨再生。通过与 MC3T3-E1 细胞的良好相互作用证明了复合支架的细胞相容性。将羟基磷灰石添加到 CD 基聚氨酯支架中可以提高细胞附着、良好的扩展形态和更高的增殖率。羟基磷灰石-聚氨酯支架具有应用于骨修复和再生的潜力。
