Analytical & Testing Center, Hainan University, Haikou, P.R. China.
Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, P.R. China.
J Biomater Sci Polym Ed. 2023 May;34(7):845-859. doi: 10.1080/09205063.2022.2145871. Epub 2022 Nov 15.
The present work is intended to provide a base for further investigation of the composite scaffolds for bone tissue engineering, and whitlockite/polyurethane (WH/PU) scaffolds, in particular. WH CaMg(HPO)(PO) was successfully prepared by means of a chemical reaction between Ca(OH), Mg(OH) and HPO. WH/PU scaffolds were synthesized polymerization. Synthesized WH particles and WH/PU composite scaffolds were characterized using FTIR, XRD, SEM and EDS. The porosity of scaffolds was calculated by the liquid displacement method. The water contact angle of scaffolds was tested. Mechanical characterization of WH/PU composite scaffolds was evaluated according to monotonic and cyclic compression examination. MC3T3-E1 cells were employed to evaluate the cytocompatibility of scaffolds. The results showed that WH and PU were completely integrated into composite biomaterials. The maximum compressive strength and elastic modulus of WH/PU composite scaffold reached up to 5.2 and 14.1 MPa, respectively. WH/PU composite scaffold had maximum 73% porosity. The minimum contact angle of WH/PU composite scaffold was 89.16°. WH/PU composite scaffolds have a good elasticity. Cyclic compression tests showed that scaffold could recover 90% of its original shape 1 h after removing the load. WH/PU composite scaffolds exhibited a high affinity to MC3T3-E1 cells. WH/PU composite scaffolds significantly promoted proliferation and alkaline phosphatase activity of MC3T3-E1 cells when compared to those grown on tissue culture well plates. It is suggested that the WH/PU scaffolds might be suitable for the application of bone tissue engineering.
本工作旨在为进一步研究用于骨组织工程的复合支架提供基础，特别是针对水钙硅石/聚氨酯（WH/PU）支架。WH CaMg(HPO)(PO) 通过 Ca(OH)、Mg(OH) 和 HPO 之间的化学反应成功制备。WH/PU 支架通过聚合反应合成。通过使用傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和能量色散光谱（EDS）对 WH 颗粒和 WH/PU 复合支架进行了表征。通过液体置换法计算了支架的孔隙率。测试了支架的水接触角。根据单调和循环压缩试验评估了 WH/PU 复合支架的机械特性。使用 MC3T3-E1 细胞评估了支架的细胞相容性。结果表明，WH 和 PU 完全整合到复合生物材料中。WH/PU 复合支架的最大压缩强度和弹性模量分别达到 5.2 和 14.1 MPa。WH/PU 复合支架的最大孔隙率为 73%。WH/PU 复合支架的最小接触角为 89.16°。WH/PU 复合支架具有良好的弹性。循环压缩试验表明，支架在去除负载 1 小时后可恢复其原始形状的 90%。WH/PU 复合支架对 MC3T3-E1 细胞具有高亲和力。与在组织培养孔板上生长的细胞相比，WH/PU 复合支架显著促进了 MC3T3-E1 细胞的增殖和碱性磷酸酶活性。这表明 WH/PU 支架可能适用于骨组织工程的应用。
