Hofírek B, Horín P, Machatková M, Granátová M, Mádr V
Vet Med (Praha). 1986 Oct;31(10):577-85.
BLV detection by the syncytial test was performed in 27 heifers experimentally and naturally infected by the enzootic bovine leukosis virus (BLV). The presence of BLV was demonstrated in 94.7% of the animals. The bovine foetal spleen cells (FBS) were found to be suitable for the syncytial test. Positive animals not reacting to infection by the production of anti-BLV antibodies were identified during the syncytial-test investigation. The importance of this finding for the programme of controlling enzootic bovine leukosis on farms is discussed. As suggested by the results, temporary occurrence of anti-BLV antibodies followed by their disappearance can be observed together with a negative result of the syncytial test in some circumstances. The discussion deals with the problems of the determination of anti-BLV antibodies in milk, and/or milk secretion, by the ELISA method.
通过合胞体试验对27头经实验感染和自然感染地方流行性牛白血病病毒(BLV)的小母牛进行了BLV检测。94.7%的动物检测出存在BLV。发现牛胎儿脾细胞(FBS)适用于合胞体试验。在合胞体试验调查期间,发现了一些对感染无抗BLV抗体产生反应的阳性动物。讨论了这一发现对农场地方流行性牛白血病防控计划的重要性。结果表明,在某些情况下,可观察到抗BLV抗体暂时出现后又消失,同时合胞体试验结果为阴性。讨论了通过ELISA方法测定牛奶和/或乳汁中抗BLV抗体的问题。