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[使用合胞体试验检测地方流行性牛白血病病毒]

[Detection of enzootic bovine leukemia virus using the syncytium test].

作者信息

Hofírek B, Horín P, Machatková M, Granátová M, Mádr V

出版信息

Vet Med (Praha). 1986 Oct;31(10):577-85.

PMID:3022450
Abstract

BLV detection by the syncytial test was performed in 27 heifers experimentally and naturally infected by the enzootic bovine leukosis virus (BLV). The presence of BLV was demonstrated in 94.7% of the animals. The bovine foetal spleen cells (FBS) were found to be suitable for the syncytial test. Positive animals not reacting to infection by the production of anti-BLV antibodies were identified during the syncytial-test investigation. The importance of this finding for the programme of controlling enzootic bovine leukosis on farms is discussed. As suggested by the results, temporary occurrence of anti-BLV antibodies followed by their disappearance can be observed together with a negative result of the syncytial test in some circumstances. The discussion deals with the problems of the determination of anti-BLV antibodies in milk, and/or milk secretion, by the ELISA method.

摘要

通过合胞体试验对27头经实验感染和自然感染地方流行性牛白血病病毒(BLV)的小母牛进行了BLV检测。94.7%的动物检测出存在BLV。发现牛胎儿脾细胞(FBS)适用于合胞体试验。在合胞体试验调查期间,发现了一些对感染无抗BLV抗体产生反应的阳性动物。讨论了这一发现对农场地方流行性牛白血病防控计划的重要性。结果表明,在某些情况下,可观察到抗BLV抗体暂时出现后又消失,同时合胞体试验结果为阴性。讨论了通过ELISA方法测定牛奶和/或乳汁中抗BLV抗体的问题。

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