Amborski G F, Lo J L, Seger C L
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Baton Rouge, LA.
Vet Microbiol. 1989 Jul;20(3):247-53. doi: 10.1016/0378-1135(89)90048-5.
Individual experimental animals used in our studies on bovine leukemia virus (BLV) are routinely screened for the presence of antibodies to the three bovine lymphotropic retroviruses. We utilized these screening methods to examine frozen sera from eight herds for antibodies to BLV, bovine visna virus (BVV) and bovine syncytial virus (BSV). Serum samples from 235 animals in four dairy and four beef herds were analyzed. Detection methods used included indirect fluorescent antibody tests of virus-infected cell cultures (BLV, BSV, BVV) and agar gel immunodiffusion (BLV). Sera from the BLV-infected animals in the dairy herds showed the highest single (50%, 49/97) and multiple (30%, 29/97) infections compared with 5% (7/138) and less than 1% (1/138), respectively in the beef herds. Single BVV infections were not detected in the dairy herds, but 11% (11/97) of the sera contained antibodies to BVV plus BLV or BSV. Five sera from beef cattle had antibodies only to BVV and four were obtained from one herd. Only one beef serum of the 138 tested demonstrated multiple antibodies (BLV, BVV).
在我们对牛白血病病毒（BLV）的研究中，所用的实验动物个体都会定期接受针对三种嗜牛性逆转录病毒抗体的筛查。我们利用这些筛查方法检测了来自八个牛群的冷冻血清，以查找针对BLV、牛维斯纳病毒（BVV）和牛合胞体病毒（BSV）的抗体。对四个奶牛群和四个肉牛群中的235只动物的血清样本进行了分析。所采用的检测方法包括对病毒感染细胞培养物进行间接荧光抗体检测（针对BLV、BSV、BVV）以及琼脂凝胶免疫扩散法（针对BLV）。与肉牛群中分别为5%（7/138）和不到1%（1/138）的感染率相比，奶牛群中感染BLV的动物血清显示出最高的单一感染率（50%，49/97）和多重感染率（30%，29/97）。在奶牛群中未检测到单一的BVV感染，但11%（11/97）的血清含有针对BVV加BLV或BSV的抗体。五份肉牛血清仅含有针对BVV的抗体，其中四份来自同一个牛群。在138份检测的肉牛血清中，只有一份显示有多种抗体（BLV、BVV）。
