Department of Pathology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Department of Pathology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Oncol Rep. 2018 Nov;40(5):2683-2689. doi: 10.3892/or.2018.6705. Epub 2018 Sep 13.
Metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1) is a long non‑coding RNA (lncRNA) that has an oncogenic role in some types of cancers, uncluding breast cancer (BC). To investigate the role of MALAT1 in human BC progression, we detected MALAT1 expression levels based on tissue samples from 20 BC cases and 20 healthy controls and found MALAT1 expression levels to be significantly high (P<0.05). Then, we knocked down endogenous MALAT1 in MCF‑7 cells using MALAT1 short hairpin RNA (shRNA). The results revealed that MALAT1 knockdown could significantly inhibit proliferation, migration, and tube formation in vitro. In addition, miR‑145 expression inversely changed in BC tissue cases. Furthermore, knockdown of endogenous MALAT1 significantly increased miR‑145 levels in MCF‑7 cells. This finding indicated an interaction between MALAT1 and miR‑145. In addition, knockdown of MALAT1 significantly reduced the expression of vascular endothelial growth factor in MCF‑7 cells. This outcome revealed that MALAT1 promoted angiogenesis in BC, which may be related to the expression of miR‑145.
转移相关肺腺癌转录物 1（MALAT1）是一种长链非编码 RNA（lncRNA），在包括乳腺癌（BC）在内的一些类型的癌症中具有致癌作用。为了研究 MALAT1 在人 BC 进展中的作用，我们根据 20 例 BC 病例和 20 例健康对照的组织样本检测了 MALAT1 的表达水平，发现 MALAT1 的表达水平显著升高（P<0.05）。然后，我们使用 MALAT1 短发夹 RNA（shRNA）在 MCF-7 细胞中敲低内源性 MALAT1。结果表明，MALAT1 敲低可显著抑制细胞的体外增殖、迁移和管形成。此外，BC 组织病例中 miR-145 的表达呈反向变化。此外，内源性 MALAT1 的敲低显著增加了 MCF-7 细胞中 miR-145 的水平。这一发现表明 MALAT1 和 miR-145 之间存在相互作用。此外，MALAT1 的敲低显著降低了 MCF-7 细胞中血管内皮生长因子的表达。这一结果表明 MALAT1 促进了 BC 的血管生成，这可能与 miR-145 的表达有关。
