Jia Zhen, Chen Zhijun, Xu Hongfei, Armah Malik Akuffu, Teng Peng, Li Weidong, Jian Dongdong, Ma Liang, Ni Yiming
Department of Cardio-Thoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang - China.
Department of Cardio-Thoracic Surgery, Zhoushan Hospital, Zhejiang - China.
Arq Bras Cardiol. 2018 Jun;110(6):568-576. doi: 10.5935/abc.20180088.
The cardiac hypertrophy (CH) model for mice has been widely used, thereby providing an effective research foundation for CH exploration.
To research the effects of CH modeling under abdominal aortic constriction (AAC) using different needles and weights in mice.
Four needles with different external diameters (0.35, 0.40, 0.45, and 0.50 mm) were used for AAC. 150 male C57BL/6 mice were selected according to body weight (BW) and divided into 3 weight levels: 18 g, 22 g, and 26 g (n = 50 in each group). All weight levels were divided into 5 groups: a sham group (n = 10) and 4 AAC groups using 4 ligation intensities (n = 10 per group). After surgery, survival rates were recorded, echocardiography was performed, hearts were dissected and used for histological detection, and data were statistically analyzed, P < 0.05 was considered statistically significant.
All mice died in the following AAC groups: 18g/0.35 mm, 22 g/0.35 mm, 26 g/0.35 mm, 22 g/0.40 mm, and 26 g/0.40 mm. All mice with AAC, those ligated with a 0.50-mm needle, and those that underwent sham operation survived. Different death rates occurred in the following AAC groups: 18 g/0.40 mm, 18 g/0.45 mm, 18 g/0.50 mm, 22 g/45 mm, 22 g/0.50 mm, 26 g/0.45 mm, and 26 g/0.50 mm. The heart weight/body weight ratios (5.39 ± 0.85, 6.41 ± 0.68, 4.67 ± 0.37, 5.22 ± 0.42, 4.23 ± 0.28, 5.41 ± 0.14, and 4.02 ± 0.13) were significantly increased compared with those of the sham groups for mice with the same weight levels.
A 0.45-mm needle led to more obvious CH than did 0.40-mm and 0.50-mm needles and caused extraordinary CH in 18-g mice.
小鼠心脏肥大（CH）模型已被广泛应用，为CH的研究提供了有效的基础。
研究在小鼠腹主动脉缩窄（AAC）模型中使用不同型号针头和重量进行CH建模的效果。
使用4种不同外径（0.35、0.40、0.45和0.50毫米）的针头进行AAC。根据体重（BW）选择150只雄性C57BL/6小鼠，并分为3个体重水平：18克、22克和26克（每组n = 50）。所有体重水平均分为5组：假手术组（n = 10）和4个AAC组，采用4种结扎强度（每组n = 10）。手术后，记录存活率，进行超声心动图检查，解剖心脏用于组织学检测，并对数据进行统计分析，P < 0.05被认为具有统计学意义。
以下AAC组的所有小鼠均死亡：18g/0.35毫米、22g/0.35毫米、26g/0.35毫米、22g/0.40毫米和26g/0.40毫米。所有接受AAC的小鼠、用0.50毫米针头结扎的小鼠以及接受假手术的小鼠均存活。以下AAC组出现不同的死亡率：18g/0.40毫米、18g/0.45毫米、18g/0.50毫米、22g/45毫米、22g/0.50毫米、26g/0.45毫米和26g/0.50毫米。与相同体重水平假手术组相比，心脏重量/体重比（5.39±0.85、6.41±0.68、4.67±0.37、5.22±0.42、4.23±0.28、5.41±0.14和4.02±0.13）显著增加。
与0.40毫米和0.50毫米针头相比，0.45毫米针头导致的CH更明显，并在18克小鼠中引起显著的CH。
