Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, PR China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China.
Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, PR China.
Int J Biol Macromol. 2019 Feb 1;122:1313-1320. doi: 10.1016/j.ijbiomac.2018.09.091. Epub 2018 Sep 15.
Hygrophorus russula (Schaeff.) Kauffman is an edible ectomycorrhizal fungus that is widely distributed in the world. In this study, the mitogenome of H. russula was sequenced and assembled. The mitogenome of H. russula is composed of circular DNA molecules, with a total size of 55,769 bp. Further analysis indicated that the frequent use of A and T in codons contributes to the high AT content (80.87%) in the H. russula mitogenome. Comparative analysis indicated that the length and base composition of the core protein-encoding genes, and the number of tRNA genes in the H. russula mitogenome varied from that of other Agaricales mitogenomes. Gene arrangement analysis revealed a novel gene order in the H. russula mitogenome. In addition, the expansion of the mitogenome in Agaricales was found to be closely related to the increase in the number of introns. Phylogenetic analysis of the combined mitochondrial gene set showed strong support for tree topologies, and H. russula was determined to be relatively distant from other Agaricales species. This study is the first report on the mitogenome of a member of genus Hygrophorus as well as family Hygrophoraceae, which improves our understanding of mitochondrial differentiation and evolution in the important ectomycorrhizal fungi Hygrophorus species.
红汁乳菇（Schaeff.）Kauffman 是一种可食用的外生菌根真菌，广泛分布于世界各地。本研究对红汁乳菇的线粒体基因组进行了测序和组装。红汁乳菇的线粒体基因组由环状 DNA 分子组成，大小为 55769bp。进一步分析表明，密码子中 A 和 T 的频繁使用导致红汁乳菇线粒体基因组中 AT 含量较高（80.87%）。比较分析表明，红汁乳菇线粒体基因组中核心蛋白编码基因的长度和碱基组成以及 tRNA 基因的数量与其他伞菌目线粒体基因组有所不同。基因排列分析揭示了红汁乳菇线粒体基因组中的一种新的基因排列。此外，发现 Agaricales 中线粒体基因组的扩张与内含子数量的增加密切相关。基于联合线粒体基因数据集的系统发育分析为树拓扑结构提供了强有力的支持，并且确定红汁乳菇与其他伞菌目物种相对较远。本研究首次报道了 Hygrophorus 属和 Hygrophoraceae 科成员的线粒体基因组，这提高了我们对重要外生菌根真菌 Hygrophorus 物种中线粒体分化和进化的理解。
