Center for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, Jiangsu, 215123, PR China.
Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, Jiangsu, 215123, PR China.
Int J Biochem Cell Biol. 2018 Nov;104:66-72. doi: 10.1016/j.biocel.2018.09.005. Epub 2018 Sep 15.
The aim of this study was to construct DNA methylation-lncRNA-mRNA interaction trios in peripheral blood mononuclear cells. We first conducted eQTL analyses using genome-wide methylation, lncRNA and mRNA expression data from 43 Chinese females. Next, causal inference test (CIT) was used to detect the lncRNA mediation effects on methylation and mRNA. Methylation-lncRNA cis-eQTL analysis identified 11 significant cis-methylation-lncRNA pairs. Combined with the results from the next lncRNA-mRNA eQTL and methylation-mRNA eQTL analyses, the 11 significant pairs and their corresponding 11,204 target e-mRNAs formed 12,245 trios. Further CIT identified six lncRNAs as mediators in regulating the corresponding pairs between methylation and mRNA. This study detected lncRNAs with mediation effects on the correlations between DNA methylations and a large number of mRNAs.
本研究旨在构建外周血单核细胞中的 DNA 甲基化-长链非编码 RNA-mRNA 相互作用三联体。我们首先使用来自 43 位中国女性的全基因组甲基化、lncRNA 和 mRNA 表达数据进行 eQTL 分析。接下来，使用因果推断测试（CIT）检测 lncRNA 对甲基化和 mRNA 的中介效应。甲基化-lncRNA 的 cis-eQTL 分析鉴定出 11 个显著的 cis-甲基化-lncRNA 对。结合下一个 lncRNA-mRNA eQTL 和甲基化-mRNA eQTL 分析的结果，这 11 个显著对及其相应的 11,204 个靶标 e-mRNAs 形成了 12,245 个三联体。进一步的 CIT 确定了 6 个 lncRNA 作为调节 DNA 甲基化和大量 mRNAs 之间相关性的中介物。本研究检测到了在 DNA 甲基化与大量 mRNAs 的相关性中具有中介效应的 lncRNA。
