Muthmainnah C R, Eriani K, Hasri I, Irham M, Batubara A S, Muchlisin Z A
Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Faculty of Sciences, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Theriogenology. 2018 Dec;122:30-34. doi: 10.1016/j.theriogenology.2018.08.024. Epub 2018 Sep 7.
The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50 mg L. Next, the sperm thawed at 39-40 °C for 3 min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6 h after fertilization, while the hatching rate was examined 60 h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteochilus vittatus eggs (P < 0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30 mgL of glutathione (63.00 ± 5.89), however, this value was not significantly different from using concentration of 10 and 20 mg L. A higher fertilization rate was produced using glutathione concentration of 50 mg L (51.33 ± 17.01); however, it was also not significantly different from the concentration of 20, 30 and 40 mgL. In addition, a higher hatching rate was also recorded using glutathione concentration of 50 mg L (40.33 ± 12.89), this value was not significantly different from using 40 mgL glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40 mg L of diluent.
本研究的目的是确定谷胱甘肽在苏氏无须魮（Osteochilus vittatus）精子冷冻保存中的最佳浓度。采用完全随机设计（CRD），有6个处理组，3次重复。苏氏无须魮精子以1:20（精子:稀释液）的比例在稀释液中稀释，然后分别添加浓度为0、10、20、30、40和50 mg/L的谷胱甘肽。接下来，精子在39 - 40°C解冻3分钟，并与随机选取的100枚卵混合。受精后6小时观察受精卵的成功情况，受精后60小时检查孵化率。方差分析表明，添加谷胱甘肽对苏氏无须魮（Osteochilus vittatus）卵的精子活力、受精率和孵化率有显著影响（P < 0.05）。通过对新鲜精子的分类，添加30 mg/L谷胱甘肽时记录到较高的精子活力率（63.00 ± 5.89），然而，该值与使用10和20 mg/L浓度时无显著差异。使用50 mg/L谷胱甘肽浓度时产生了较高的受精率（51.33 ± 17.01）；然而，它与20、30和40 mg/L浓度也无显著差异。此外，使用50 mg/L谷胱甘肽浓度时也记录到较高的孵化率（40.33 ± 12.89），该值与使用4- 0 mg/L谷胱甘肽时无显著差异。因此，得出结论：谷胱甘肽的最佳浓度为稀释液40 mg/L。
