Research and Development of Functional Food Centre (CIDAF), Health Science Technological Park, Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Research and Development of Functional Food Centre (CIDAF), Health Science Technological Park, Granada, Spain.
J Pharm Biomed Anal. 2019 Jan 5;162:82-90. doi: 10.1016/j.jpba.2018.09.021. Epub 2018 Sep 11.
Systemic Sclerosis (SSc) is a chronic autoimmune disease whose origin and pathogenesis are not yet well known. Recent studies are allowing a better definition of the disease. However, few studies have been performed based on metabolomics. In this way, this study aims to find altered metabolites in SSc patients in order to improve their diagnosis, prognosis and treatment. For that, 59 SSc patients and 28 healthy volunteers participated in this study. Urine and plasma samples were analysed by a fingerprinting metabolomic approach based on HPLC-ESI-QTOF-MS. We observed larger differences in urine than plasma metabolites. The main deregulated metabolic families in urine were acylcarnitines, acylglycines and metabolites derived from amino acids, specifically from proline, histidine and glutamine. These results indicate perturbations in fatty acid beta oxidation and amino acid pathways in scleroderma patients. On the other hand, the main plasma biomarker candidate was 2-arachidonoylglycerol, which is involved in the endocannabinoid system with potential implications in the induction and propagation of systemic sclerosis and autoimmunity.
系统性硬化症（SSc）是一种慢性自身免疫性疾病，其起源和发病机制尚不清楚。最近的研究使我们对该疾病有了更好的定义。然而，基于代谢组学的研究很少。通过这种方式，本研究旨在寻找 SSc 患者中代谢物的变化，以改善其诊断、预后和治疗。为此，59 名 SSc 患者和 28 名健康志愿者参与了这项研究。通过基于 HPLC-ESI-QTOF-MS 的指纹代谢组学方法分析尿液和血浆样本。我们观察到尿液中代谢物的差异大于血浆中的代谢物。尿液中主要失调的代谢家族是酰基肉碱、酰基甘氨酸和氨基酸衍生的代谢物，特别是脯氨酸、组氨酸和谷氨酰胺。这些结果表明系统性硬化症患者的脂肪酸β氧化和氨基酸途径受到干扰。另一方面，血浆生物标志物候选物主要是 2-花生四烯酸甘油，它参与内源性大麻素系统，对系统性硬化症和自身免疫的诱导和传播具有潜在影响。
