College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Plant Physiol Biochem. 2018 Nov;132:238-248. doi: 10.1016/j.plaphy.2018.09.012. Epub 2018 Sep 11.
Abiotic stress causes various negative impacts on plants, such as water loss, reactive oxygen species (ROS) accumulation and decreased photosynthesis. R2R3-MYB transcription factors (TFs) play crucial roles in the response of plants to abiotic stress. However, their functions in Tartary buckwheat, a strongly abiotic and resistant coarse cereal, haven't been fully investigated. In this paper, we report that a R2R3-MYB from Tartary buckwheat, FtMYB13, is not an activator of transcriptional activity but is located in the nucleus. Moreover, compared to the wild type (WT), transgenic Arabidopsis overexpressing FtMYB13 had a lower sensitivity to ABA and caused improved drought/salt tolerance, which was attributed to the higher proline content, greater photosynthetic efficiency, higher transcript abundance of some stress-related genes and the smaller amount of reactive oxygen species (ROS) and malondialdehyde (MDA) in the transgenic lines compared to WT. Consequently, our work indicates that FtMYB13 is involved in mediating plant responses to ABA, as well as salt and drought.
非生物胁迫会对植物造成各种负面影响，如水分流失、活性氧（ROS）积累和光合作用下降。R2R3-MYB 转录因子（TFs）在植物应对非生物胁迫中起着至关重要的作用。然而，它们在荞麦，一种抗逆性很强的粗粮中的功能尚未被充分研究。在本文中，我们报道了荞麦中的一个 R2R3-MYB，FtMYB13，它不是转录活性的激活剂，而是位于细胞核内。与野生型（WT）相比，过表达 FtMYB13 的转基因拟南芥对 ABA 的敏感性较低，且耐旱/盐能力增强，这归因于转基因系中脯氨酸含量较高、光合作用效率较高、一些与胁迫相关基因的转录丰度较高，以及活性氧（ROS）和丙二醛（MDA）的含量较低。因此，我们的工作表明 FtMYB13 参与调节植物对 ABA 以及盐和干旱的反应。
