Gao Fei, Zhao Hai-Xia, Yao Hui-Peng, Li Cheng-Lei, Chen Hui, Wang An-Hu, Park Sang-Un, Wu Qi
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, 625014, Ya'an, Sichuan, China.
Xichang College, 615013, Xichang, Sichuan, China.
Plant Cell Rep. 2016 Jun;35(6):1385-96. doi: 10.1007/s00299-016-1971-5. Epub 2016 Mar 28.
Eight R2R3 - MYB genes in tartary buckwheat were identified, and their expression patterns were comprehensively analyzed, which reveals role in plant response to abiotic stresses. The proteins of the R2R3-MYB superfamily play key roles in the growth and development processes as well as defense responses in plants. However, their characteristics and functions have not been fully investigated in tartary buckwheat (Fagopyrum tataricum), a strongly abiotic resistant coarse cereal. In this article, eight tartary buckwheat R2R3-MYB genes were isolated with full-length cDNA and DNA sequences. Phylogenetic analysis of the members of the R2R3-MYB superfamily between Arabidopsis and tartary buckwheat revealed that the assumed functions of the eight tartary buckwheat R2R3-MYB proteins are divided into five Arabidopsis functional subgroups that are involved in abiotic stress. Expression analysis during abiotic stress and exogenous phytohormone treatments identified that the eight R2R3-MYB genes responded to one or more treatments. This study is the first comprehensive analysis of the R2R3-MYB gene family in tartary buckwheat under abiotic stress.
在苦荞中鉴定出8个R2R3 - MYB基因，并对其表达模式进行了全面分析，揭示了它们在植物对非生物胁迫响应中的作用。R2R3 - MYB超家族蛋白在植物的生长发育过程以及防御反应中发挥关键作用。然而，在具有较强非生物抗性的粗粮苦荞（鞑靼荞麦）中，它们的特性和功能尚未得到充分研究。在本文中，分离出了8个具有全长cDNA和DNA序列的苦荞R2R3 - MYB基因。对拟南芥和苦荞之间的R2R3 - MYB超家族成员进行系统发育分析表明，8个苦荞R2R3 - MYB蛋白的假定功能分为参与非生物胁迫的5个拟南芥功能亚组。对非生物胁迫和外源植物激素处理期间的表达分析表明，这8个R2R3 - MYB基因对一种或多种处理有响应。本研究是首次在非生物胁迫下对苦荞中R2R3 - MYB基因家族进行全面分析。
