干旱胁迫下金银花MYB转录因子的表达分析与功能研究
Expression analysis and functional study of honeysuckle MYB transcription factors under drought stress.
作者信息
Tang Zhiqiang, Wang Jinghua, Li Runzhu, Tang Yanan, Zhang Yongqing, Pu Gaobin
机构信息
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.
Shandong Provincial Collaborative Innovation Center for Quality Control of Traditional Chinese Medicine and Construction of the Whole Industrial Chain, Jinan, 250355, Shandong, China.
出版信息
Sci Rep. 2025 Apr 28;15(1):14843. doi: 10.1038/s41598-025-99946-1.
In this study, 356 MYB transcription factors were identified from the genome of honeysuckle, and combined with transcriptome data analysis, 104 of them were found to respond to drought stress. Through phylogenetic analysis, qRT-PCR analysis and correlation analysis of chemical components, three target genes LjMYB3, LjMYB8 and LjMYB63 were screened from these 104 MYB transcription factors and overexpressed in Arabidopsis thaliana. The results showed that the drought resistance, total flavonoid content and flavonoid biosynthesis-related gene expression levels of transgenic Arabidopsis were higher than those of wild-type Arabidopsis.Under drought stress, MYB transcription factors can activate the expression of drought resistance-related genes, regulate key genes in the flavonoid synthesis pathway, and promote the accumulation of flavonoids. This study promotes the research on transcriptional regulation of honeysuckle MYB transcription factors and flavonoid biosynthesis and lays a foundation for unraveling the drought resistance mechanism in honeysuckle.
在本研究中，从金银花基因组中鉴定出356个MYB转录因子，并结合转录组数据分析，发现其中104个对干旱胁迫有响应。通过系统发育分析、qRT-PCR分析以及化学成分相关性分析，从这104个MYB转录因子中筛选出3个靶基因LjMYB3、LjMYB8和LjMYB63，并在拟南芥中过表达。结果表明，转基因拟南芥的抗旱性、总黄酮含量以及黄酮生物合成相关基因的表达水平均高于野生型拟南芥。在干旱胁迫下，MYB转录因子可激活抗旱相关基因的表达，调控黄酮合成途径中的关键基因，并促进黄酮类物质的积累。本研究推动了金银花MYB转录因子转录调控及黄酮生物合成的研究，为揭示金银花的抗旱机制奠定了基础。
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