Fini M E, Gross R H, Brinckerhoff C E
Arthritis Rheum. 1986 Nov;29(11):1301-15. doi: 10.1002/art.1780291102.
To provide tools for understanding collagenase gene expression in rheumatoid arthritis, we have isolated and characterized genomic clones for rabbit synovial cell collagenase. These clones represent 2 types of collagenase gene, at least 1 of which is transcribed in synovial fibroblasts. By examining the rabbit genome in situ, we provide evidence that there are only 2 different synovial cell collagenase genes found in a haploid genome. Amplification of these genes is not a mechanism for collagenase messenger RNA induction by phorbol esters.
为了提供理解类风湿关节炎中胶原酶基因表达的工具，我们分离并鉴定了兔滑膜细胞胶原酶的基因组克隆。这些克隆代表了2种胶原酶基因类型，其中至少1种在滑膜成纤维细胞中被转录。通过原位检测兔基因组，我们提供证据表明在单倍体基因组中仅发现2种不同的滑膜细胞胶原酶基因。这些基因的扩增不是佛波酯诱导胶原酶信使核糖核酸的机制。
