Laboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, France.
Laboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord des Hospices Civils de Lyon, 69317 CEDEX 04 Lyon, France.
Viruses. 2018 Sep 17;10(9):504. doi: 10.3390/v10090504.
In 2009, the co-circulation of H5N1 and H1N1pdm09 raised concerns that a reassortment event may lead to highly pathogenic influenza strains. H1N1pdm09 and H5N1 are able to infect the same target cells of the lower respiratory tract. To investigate the capacity of the emergence of reassortant viruses, we characterized viruses obtained from the co-infection of cells with H5N1 (A/Turkey/13/2006) and H1N1pdm09 (A/Lyon/969/2009 H1N1). In our analysis, all the screened reassortants possessed the PB2, HA, and NP segments from H5N1 and acquired one or two of the H1N1pdm09 segments. Moreover, the in vivo infections showed that the acquisition of the NS segment from H1N1pdm09 increased the virulence of H5N1 in mice. We conclude, therefore, that reassortment can occur between these two viruses, even if this process has never been detected in nature.
2009 年，H5N1 和 H1N1pdm09 的共同流行引起了人们的担忧，即重组事件可能导致高致病性流感株的出现。H1N1pdm09 和 H5N1 能够感染下呼吸道的相同靶细胞。为了研究重组病毒出现的能力，我们对从 H5N1（A/Turkey/13/2006）和 H1N1pdm09（A/Lyon/969/2009 H1N1）共感染细胞中获得的病毒进行了特征分析。在我们的分析中，所有筛选出的重组体均具有来自 H5N1 的 PB2、HA 和 NP 片段，并获得了一个或两个 H1N1pdm09 片段。此外，体内感染表明，从 H1N1pdm09 中获得 NS 片段会增加 H5N1 在小鼠中的毒力。因此，我们得出结论，即使在自然界中从未检测到这种情况，这两种病毒之间也可能发生重组。
