Subramoniam A, Padh H, Aleo J J
Biochem Med Metab Biol. 1986 Feb;35(1):65-71. doi: 10.1016/0885-4505(86)90059-9.
Polymyxin B sulfate treatment induced an increase in calcium influx in mouse fibroblasts (3T6) and normal human skin fibroblasts. This increase in calcium influx occurred in a dose- and time-dependent fashion and was dependent on pH but independent of the electrochemical gradient of calcium across the plasma membrane. This effect was prevented when cycloheximide (20 micrograms/ml) was added with polymyxin B sulfate. Addition of actinomycin D (2 micrograms/ml) also remarkably reduced this effect. In view of these findings, it is possible that polymyxin B sulfate therapy-induced side effects, such as neuromuscular blockade and kidney dysfunction, are conditional and may be due to an increase in calcium influx.
硫酸多粘菌素B处理可诱导小鼠成纤维细胞（3T6）和正常人皮肤成纤维细胞的钙内流增加。这种钙内流的增加呈剂量和时间依赖性，且依赖于pH值，但与钙跨质膜的电化学梯度无关。当加入环己酰亚胺（20微克/毫升）与硫酸多粘菌素B时，这种效应被阻止。加入放线菌素D（2微克/毫升）也显著降低了这种效应。鉴于这些发现，硫酸多粘菌素B治疗引起的副作用，如神经肌肉阻滞和肾功能障碍，可能是有条件的，并且可能是由于钙内流增加所致。
