Ha Youngsil, Thienes Cortlandt P, Agapov Alexander A, Laznicka Amanda V, Han Sukkyun, Nadala Cesar, Samadpour Mansour
Molecular Epidemiology, Inc., 15300 Bothell Way N.E., Lake Forest Park, WA 98155.
IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way N.E., Lake Forest Park, WA 98155.
J AOAC Int. 2018 Jul 6. doi: 10.5740/jaoacint.18-0128.
Concerns about the contamination of meat products with undeclared meats and new regulations for the declaration of meat adulterants have established the need for a sensitive test to detect meat adulteration. To address this need, Microbiologique, Inc. has developed ELISA assays that can detect the presence of pork, horse, beef, chicken, turkey, and goat meat adulterants to 0.1% (w/w) and a deoxyribonucleic acid (DNA) lateral flow assay for pork, horse, beef, chicken, turkey, goat, and lamb adulterants to 0.1% (w/w). We compared the results of the DNA lateral flow assay to the ELISA assays. ELISA and DNA lateral flow assays were performed on the same spiked meat samples, prepared meats, and pet foods. Both the DNA lateral flow and the ELISA assays were sensitive to 0.1% meat adulterant, and the agreement between the DNA lateral flow and ELISA assays for spiked samples, prepared meat, and pet foods was 100%. Based on the 100% concordance between the two assay formats, the choice between the two is dependent on whether quantitation is desired, which assay is more familiar to the particular laboratory, availability of the required equipment, and time restrictions. The ELISA assays are less time consuming, taking about 1.5 h, compared with about 2.5 h for the DNA lateral flow assay. Because the DNA lateral flow test detects seven species in one test, it can be more cost effective when the potential adulterant is not known, while the ELISA may be better for quantification.
对肉制品被未申报肉类污染的担忧以及肉类掺假物申报的新规定，使得有必要开展一项灵敏的检测来检测肉类掺假情况。为满足这一需求，微生物公司开发了酶联免疫吸附测定（ELISA）法，该方法能够检测出猪肉、马肉、牛肉、鸡肉、火鸡肉和羊肉掺假物，检测限为0.1%（重量/重量）；还开发了一种用于检测猪肉、马肉、牛肉、鸡肉、火鸡肉、羊肉和羔羊肉掺假物的脱氧核糖核酸（DNA）侧向流动分析法，检测限同样为0.1%（重量/重量）。我们将DNA侧向流动分析法的结果与ELISA法的结果进行了比较。ELISA法和DNA侧向流动分析法均应用于相同的加标肉类样品、加工肉类和宠物食品。DNA侧向流动分析法和ELISA法对0.1%的肉类掺假物均敏感，对于加标样品、加工肉类和宠物食品，DNA侧向流动分析法与ELISA法的结果一致性为100%。基于两种检测方法100%的一致性，在两者之间进行选择取决于是否需要定量、特定实验室对哪种检测方法更熟悉、所需设备是否可用以及时间限制。ELISA法耗时较短，约需1.5小时，而DNA侧向流动分析法约需2.5小时。由于DNA侧向流动检测一次能检测七种物种，当潜在掺假物未知时，它可能更具成本效益，而ELISA法可能更适合定量分析。
